| ObjectiveTo observe the effect of Qi Long Yi Shen decoction on the level of serum Cys C,Hb Alc,um Alb and hemorheological indexes such as whole blood high shear viscosity(Hηb),whole blood low shear viscosity(Lηb), plasma viscosity(ηp), erythrocyte aggregation index(EAI), fibrinogen(FIB), red blood cells deposited(Hct) in rats with early diabetic nephropayhy between pre-treatment and post-treatment. And to observe the effect of Qi Long Yi Shen decoction on their renal pathological changes at the same time.Therefore this study was designed to explore whether Qi Long Yi Shen decoction can effectively prevent and treat early DN rats, and provide experimental basis for clinical application of Qi Long Yi Shen decoction.And also to provide a reference for the similar research prescriptions.MethodsSPF male Wistar rats weighted between 180 g to 220g(n=60)were selected as exprimental animals to executed this research. 10 rats were choosed randomly as normal control group(group A) to fed with ordinary diet. While 50 rats were assigned to establish the Diabetic Nephropathy model, which were fed with high fat diet for 8 weeks. Then the 50 rats were fasted for 12 hours and injected with streptozotocin(STZ) in lower dose of 30mg/kg for three days,and fed with high fat diet for 3 weeks continuely to establish the Diabetic Nephropathy model. After 3 days of establishing model, rats blood were collected from tail tip to test random blood sugar. and After 3 days of establishing model, rats blood were collected from tail tip to test random blood sugar, and 24 hours urine were collected to test urine albumin excretion rate. If rats blood sugar reaches to or higher than 16.7mmol/L,urine sugar is higher than + +(including + +), urine volume increased to double, and proteinuria was appeared(24h urine protein) the Diabetic Nephropathy model was believed to be successful. Then the rats of early DN were randomly divided into model group(group B), Qi Long Yi Shen decoction high dose group(group C), middle dose group(group D), low dose group(group E), and IRBESANTAN group(group F).Subsequently, Qi Long Yi Shen decoction treatment group were administrated orally in rats model with the following doages(the high dose: 25.2 g/kg/d,low dose:6.3g/kg/d, middle dose:12.6g/kg/d)respectively,and the positive group were administrated orally with Irbesartan by the dosage of 12.6mg/kg/d.While the normal control group and model group rats were administrated orally with physiological saline as same volume every day.All rats were treated for 8 weeks. After the final gavage trearment for 12 hours, All rats were anesthetized with 10% Chloral Hydrate to collect artery blood 24 hours urine,and kidney tissue for testing. All samples were collected to observe the changes of serum Cys C,Hb Alc,um Alb level and hemorheological indexes such as Hηb,Lηb, ηp, EAI, FIB, Hct, etc to compare of the indexes between pre-treatment and post-treatment and among different groups of all groups. In addition, dyed kidney tissues were fixed with 10% formaldehyde for HE. Staining to observe the pathological changes.Results1. There were five rats died in the process of establishing, and the success rate of establishing amounts to 90%. The detection results of rats’ blood and collected urine indicated: Compared with normal control group, levels of serum Cys C, um Alb, Hb Alc and hemorheological indexes such as Hηb, Lηb, ηp, EAI, FIB, Hct, etc of Model control group and each treatment group were significantly increased, and the differences showed statistically significance(P < 0.05).2. After completing intervention treatment, the detection results of rats’ blood and collected urine indicated: compared with the model control group, levels of serum Cys C,um Alb, Hb Alc and hemorheological indexes such as Hηb, Lηb, ηp, EAI, FIB, Hct, etc decreased in different degrees, and the above indexes of high dose of Qi Long Yi Shen decoction group decreased extremely, the differences showed statistical significance(P<0.05); Compared with pre-treatment, the level all of the indexes above decreased in each treatment group in different degrees, and the above indexes of middle dose of Qi Long Yi Shen decoction group decreased extremely, which showed statistically significance(P< 0.05).3. HE staining morphology showed: Compared with normal control group, renal glomerulus were obvious hypertrophy, renal cysts are fractured, mesangial are proliferated, GBM become thickening, mesangial region are widen, and renal epithelial cells are arranged disorderly and turn to be hypertrophy and vacuolar degeneration in all the other groups. Compared with the model group, the pathological changes of Qi Long Yi Shen decoction treatment groups of high and middle dosage and irbesartan group recovered in certain extent, and only Qi Long Yi Shen decoction treatment group of high dosage changed greatly.Conclusions1. Qi Long Yi Shen decoction could obviously decrease the level of rat’s serum Cys C,Hb Alc,and um Alb, as a result, It has significant preventive and curative effects on rats with early DN.2. Compared with irbesartan, Qilong Yi Shen Decoction could reduce the indexes of blood rheology, and improve the rat blood stasis symptoms.3. Qi Long Yi Shen decoction could effectively delay the renal pathological changes of rats with DN, and it plays a protective and curative role in the treatment on rats’ kidney with DN by employing the curative law of supplementing qi and nourishing yin. |
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