Influence Of Qizhi Zhoufei Granules On P38MAPK Signaling Pathway Of The LPS Iduced Inflammatory Alveo-Lar TypeⅡ Epithelial Cells

Objective :Primary cultured of alveolar typle Ⅱ cells(AT-Ⅱ) of new born rat,using lipopolysaccharide(LPS) manufacturing cells vitro inflammation model,Qizhi Zhoufei granule interventin cells,observe the inflammartory of AT-Ⅱmorphology,the expression of gene and protein on the p38 MAPK signal pathway.To explore the action mechanism of Qizhi Zhoufei granule of chronic obstructive pulmonary lung disease(chronic obstructive pμlmonary disease, COPD),providing pharmacodynamicsto the development of research on Qizhi Zhoufei granule.Methods :Separate AT-Ⅱcells from lungs of SPF newborn Wister rat by trypsin combined collagenase digestion method,cultured in vitro after isolation and purification.Observed and identified AT-Ⅱcells through transmission electron microscope.Taken the growth state approach the same generation cells,setted the blank group,model group(LPS 20ug/ml),lose dose group(LPS 20ug/ml+ Qizhi Zhoufei granule 50ug/ml),middle dose group(LPS 20ug/ml+ Qizhi Zhoufei granule 100ug/ml),high dose group(LPS 20ug/ml+ Qizhi Zhoufei granule 200ug/ml), observed the growth state throgh electron microscope,lamellar corpuscle throgh transmission electron microscope,detected the expression og SP-B by immunohistochemical method,the gene expression level of p38、SP-B and Fas in each group by Q-PCR,the protein expression level of p38 and SP-B in each group by WBResults :1. Primary cultured AT-Ⅱcells successfully,,cells in good growth station;2. LPS established inflammtion COPD model in vitro successfully, bserved throughtransmission electron microscope,compared with blank group, Lb reduced inAT-Ⅱof model group,there was vaculor change;compared with model group, Lbreduced in AT-Ⅱof Qizhi Zhoufei granuleslose,middle,high group not significant,vaculor change lower;3. Compared with blank group,the expression level reduced of SP-B gene,p38 gene andFAS gene increased(p ﹤ 0.05); compared with model group, Qizhi Zhoufeigranuleslose,middle,high group could increased the SP-B low expression level,reduced thep38 and FAS high expression level;Conclusion:LPS intervention in primary cultured rat AT-Ⅱ cells, established inflammtion COPD model in vitro successfully,induced prolifartion of inflammatory cells,ultrastructure changes in cells, Qizhi Zhoufei granules could inhibit cells proliferation,improve ultrastructure changes, increased the SP-B expression level,regulation he p38 and FAS high expression level,reduce inflammtion,improve the biological activity of AT-Ⅱcells...