| Objective:To establish the fingerprint of Huanglian Shangqing pills, identified as much as possible the total chemical composition, combined with pattern recognition method, analysis of different manufacturers, different batches of Huanglian Shangqing pills in the quality of the information, and search for the cause of the difference of chemical composition, for the quality evaluation of Huanglian Shangqing pills on sale, at the same time provide a reference to improve the quality standard of Huanglian Shangqing pills. Methods:Using high performance liquid chromatography(HPLC) fingerprint of the sample preparation method, chromatographic conditions were investigated to establish a series of Huanglian Shangqing pills, get the fingerprint of common peaks information, and Berberine Hydrochloride, Baicalin and other eight kinds of standard identification of common peaks. The evaluation system of A for chromatographic fingerprint, to obtain the common pattern of the fingerprint and similarity calculation. Establish a suitable pattern recognition method model analysis using SIMCA 13.0 software, according to the model of the load scatter, scattered point graph and variable importance factor(VIP value), looking for the great contribution caused by the difference of chemical composition of quality components in Huanglian Shangqing pills. Results:HPLC fingerprint of Huanglian Shangqing pills was established, obtained a total of 34 peaks, identified eight kinds of chemical composition, similarity reached 0.745 ~ 0.946. PLS-DA is more suitable for the analysis of Huanglian Shangqing pills fingerprint than PCA, in the data extraction of 4 principal components, can explain the change of 73.7% X variables and 88.5% Y variables, and prediction ability has 75.6%. The samples of different manufacturers have good distinction, and to find the cause of the differences in the quality of the larger contribution of chemical composition Conclusion:The chemical composition of eight manufacturers of 22 batches of Huanglian Shangqing pills sample almost the same, that the manufacturers in the production process in accordance with the prescription of feeding. The differences of 34 common content, may be due to different production process, origin or sources of Chinese herbal medicine, etc. There was a good correlation with the content of manufacturer of components, description of the same manufacturer’s production process, origin of Chinese herbal medicines, and other more fixed sources. This experimental research method not only can be used for the quality evaluation of differences between different manufacturers of Huanglian Shangqing pills, also can be used in the same factory for quality control of different batches of products, to ensure the stability of the quality. |
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