| Buzhongyiqi is a famous prescription by extraordinarily skilled practitioner Li Dongyuan,which is composed of Radix Astragali,Rhizoma Atractylodis Macrocephalae,Radix Codonopsis,Radix Angelicae Sinensis,Pericarpium Citri Reticulata,Radix Glycyirrhizae,Radix Bupleuri,Rhizoma Cimicifugae.This experiment takes Buzhongyiqi pills as a research object and adopt the technique of many index sign composition metered fingerprint diagram quality control combine a method of Ultraviolet spectrum group method to control Buzhongyiqi pills of quality. Put great emphasis on the pole part fingerprint in pill diagram establishment and construction of its identifies mode,which is supplementary and enhanced to a method of quality control on the pill.After a great deal of literature investigation,Chapter 1 provides an overview of Chinese medicine fingerprint technology,quality control technology and pattern recognition technology in the fingerprint of traditional Chinese medicine research,,in this basis,the combined pill Buzhong Research,summed up the thesis research based on the legislation, research purpose,meaning and main content.Chapterâ…¡using UV spectrum group method of to conduct testing Buzhongyiqi pills maked by different manufacturers in the market,drawn map of the UV spectrum and investigate its quality difference.Using petroleum ether,chloroform,ethanol and distilled water to extract Buzhongyiqi pills,then the corresponding UV detection,and UV spectra obtained set of data for cluster analysis and principal component analysis. Through the UV spectrum of groups and cluster analysis of the preliminary determination can make a distinction between the quality of Buzhongyiqi pills produced by different manufacturers.Chapterâ…¢use RP-HPLC:Column,Sunfire C18 column;mobile phase of 0.05%phosphoric acid - acetonitrile(gradient elution);detection wavelength:254nm;analysis time:70min;flow rate:1.0mL / min.The results identified a total of 30 peaks,there are peaks of the relative retention time RSD values are less than 1%,single peak area greater than or equal to 10%peak area ratio is also relatively stable.It have been separated and good reproducibility of the HPLC-DAD fingerprinting.By side with the the relevance of research on fingerprint and the main fingerprint peaks were assigned,in conjunction with cluster analysis, assess samples from different manufacturers and different production Technology pills quality.Chapterâ…£base on the active ingredients in traditional Chinese medicine-based quality control.in accordance with the different chemical properties and optical properties,using diode array detector (DAD),the establishment of a multi-index components quantitative approach of Buzhongyiqi pills.use RP-HPLC method for the establishment of the method for the determination of hesperidin,glycyrrhizic acid, the establishment of multi-component quantitative indicators for the fingerprinting of the pharmaceutical quality evaluation in Buzhongyiqi pills are satisfied with the results achieved by different manufacturers.Chapterâ…¤use the methods described the above two,three,four chapters,based on the fingerprint data a large number of pills of different manufacturers and different production processes,progress characteristic pill sample qualitative and quantitative data for mathematical Statistics:Cluster Analysis System for the product cluster analysis of the computer;the establishment of the canonical discriminant function and the Fisher ' s discriminant function,build a model to identify Buzhongyiqi pills identification of unknown samples provided an accurate,fast tool.In practical applications,the sample can be described by this study,an analysis of chromatographic conditions, quantification of fingerprint characteristics will be entered into the computer calculations,can effectively differentiate between products, a more objective evaluation of the stability of the production process. This research will contribute to the quality control of Buzhongyiqi pills.
|
PDF Full Text Request