| Objectives To study the effects of the combined application of carlbachol and dietary fiber on the intestinal mucosa, the plasma endotoxin,the plasma D- lactic acid,the blood flow of intestinal mucosa and the transcutaneous oxygen partial pressure of abdomen in scald shock rats.Methods 1 With A 30% TBSAⅢ °scald Wistar rats for animal experiment model, rats were randomly divided into four groups:the group treated by physiological saline(Group A), the group treated by dietary fiber(Group B), the group treated by carlbachol(Group C),the group treated by carlbachol and dietary fiber(Group D). The rats were sacrificed at 6,12, 24, 48 and 72 hours after injury.Every time point had four rats in every group.Another four health rats were served as normal control group(Group N). Tissue samples were harvested from the small intestine to observe the changes of the morphological structure of intestinal mucosa under the light microscope.The damage of intestinal mucosa was assessed.The levels of the plasma endotoxin of portal venous blood and the levels of the plasma D-lactic acid of abdominal aorta blood were detemined.And the results were compared between different time points and groups.2 With 30%TBSAⅢ °scald Wistar rats as experimental animal model, rats were randomly divided into two group:the group treated by physiological saline(Group A), the group treated by carlbachol and dietary fiber(Group B). Then the animals were randomly divided into the time point of 0h,2h, 4h, 8h.Every time point had eight rats in every group.Another eight health rats were served as normal control group(Group N). The laser phase Doppler analyzer was applied to monitor respectively the abdominal transcutaneous oxygen partial pressure and the blood flow of intestinal mucosal at 0h, 2h, 4h, 8h after injury in rats. And the results of the three groups were compared.Results 1 The damage of the small intestine and the level of plasma endotoxin or plasma D-lactic acid of the rats in scald groups were all significantly higher than those rats in the normal group. They peaked at 12 to 24 hours and maintained higher levels than normal controls at 72 hours after the injury. Compared with the animals treated by physiological saline,the combined application of carlbachol and dietary fiber improved the morphological structure of intestinal mucosa and reduced the intestinal injury score(P<0.05); significantly reduced the levels of the plasma endotoxin(P<0.01) and the levels of the plasma D-lactic acid(P<0.01). 2 The blood flow of intestinal mucosa and the transcutaneous oxygen partial pressure of abdomen of the scalded rats were significantly lower than those in normal group.Both indicators gradually recovered as the resuscitation of shock. Compared with the animals treated by physiological saline,the combined application of carbachol and dietary fiber in scalded shock rats, significantly increased the transcutaneous oxygen partial pressure of abdomen(P<0.05) and the blood flow of the intestinal mucosa(P<0.05 or P<0.01).Conclusions 1 The damage of intestinal mucosa and increased levels of plasma endotoxin and D- lactic acid confirme that burn shock can lead to intestinal barrier dysfunction.2 The combined application of carbachol and dietary fiber can maintain the morphology and structure of intestinal mucosa of scalded rats, as well as promote the repair of damaged intestinal mucosa.3 The combined application of carbachol and dietary fiber can inhibit the increase of intestinal permeability caused by burn shock.4 The combined application of carbachol and dietary fiber can improve the hemodynamic status of scalded rats and improve the intestinal microcirculation, which is helpful to prevent intestinal injury caused by shock.5 The protective effect of the combined application on intestinal barrier in scalded,which is stronger than a single carbachol or single dietary fiber. |
PDF Full Text Request