Testosterone Propionate Induced Polycystic Ovary Syndrome Like Symptom In Mouse

Objective: To establish the polycystic ovary syndrome mice model with the symptom of polycystic ovary syndrome.Method: Five-days-old female BALB/c mice were hypodermically injected on the neck and back with androgen testosterone propionate and fed regularly until to adult. The vaginal epithelial cellular morphology cycle, sexual behavior, pregnancy ability, serum androgen levels, glucose tolerance, serum insulin levels and ovarian pathological changes were observed.Result: The challenged female mice showed infertility with the character of refusing sexual behavior and constant vaginal epithelial cell keratinization. Both the testosterone level in serum and the basic blood glucose were higher in the mice than that in the control(P<0.05). Furthermore, polycystic ovarian follicle and cystic dilatation follicle were detected in androgen treated mice. Behavior experiment shows that the model mice had high aggression performance, especially when they were disturbed by sexual assault. Gene expression levels in the hypothalamus area change significantly in the model mice.Conclusion: Hypodermic injection of postnatal female mice with testosterone propionate can sufficiently induces infertility in adult and results in loss of ovulatory cycle and abnormal ovarian pathological changes in animals. The testosterone propionate treated mouse could be used as one of animal model in the research area of polycystic ovary syndrome. In addition, we also found that the model mice with high aggression and gene expression pattern in hypothalamus area is also changed.