| Objective: in the central nervous system, Glioma is one of the common and most challenging malignant tumors in clinical,Adults multiple.Glioma is the most deadly in human tumors, in recent years in terms of treatment including surgery, radiotherapy and chemotherapy have made great progress. This article mainly through the detection of transcription factor NF-κB p65 and IL-6 expression in human glioma cells, To investigate the function of NF-κB and IL-6 and their relationship in glioma.Materials and methods: Collected in our hospital after craniotomy and resection of brain tumor tissue archival paraffin embedded specimens of 48 cases in first affiliated hospital of jiamusi university from 2009 January to 2014 March,confirmed by pathology for glioma.All specimens were not after radiotherapy, chemotherapy or immunotherapy at preoperative.Archived of 10 cases of normal brain tissue paraffin embedded root in the deep brain tissue from benign brain tumor site and excision of brain tissue injury in operation for decompression of brain tissue.Methods:The expressions of NF-κB p65 and IL-6 were detected by immunohistochemical SP,Through microscope observation of glioma cells and normal brain tissue coloration fill, statistical analysis of the results by SPSS21.0Results: HE staining results of glioma : glioma cells(WHO grade I- II grade), under the microscope we visible morphologically normal nucleus, more clearly visible nucleoli. Nuclear division, as less atypia unusual.(WHO grade III- IV level) under the microscope, we can see the normal nuclei are rare, glioma cell nucleus increased obviously, and there are certain accumulation tendency, the split image and atypia increase.1.Immunohistochemistry staining results of glioma: the expression of positive staining in 48 cases of brain glioma tissue sections of NF-κB p65 were in the cytoplasm and nucleus appear brown granules, the positive expression rate of 54.17%(26/48), while no positive expression seen in non tumor tissue sections.In I~II level the positive rate of NF-κB p65 was 33.33%(7/21), the positive rate of NF-κB p65 in III~IV level is 70.37%(19/27), there are significant differences between them(X2=6.527,p<0.05).to see a high level of glioma, the positive expression of NF-κB p65 rates are on the rise. Glioma grade and NF-κB p65 positive expression rate was positive correlation(r=0.467,P<0.01).2.Appear brown granules in the cytoplasm positive staining of IL-6 expression in 48 cases of brain glioma tissue sections, the positive expression rate of 58.33%(28/48), while no positive expression seen in non tumor tissue sections. The positive rate of IL-6 was in the I~II level 38.1%(8/21), the positive rate of IL-6 was in the III~IV level 74.07%(20/27), there is a significant difference between the two(X2=6.291,p<0.05), visible with the increased brain glioma grade, the positive expression of IL-6 is also increasing.Description of brain glioma grade and the positive expression rate of IL-6 showed a positive correlation(r=0.045,P<0.01).3.In 48 cases of IL-6 protein positive, the positive expression of NF-κB p65 protein of tissue specimens in 24 cases. Expression of IL-6 protein in 20 cases of negative, positive expression of NF- kappa Bp65 protein has 2 columns. showed close relationship between NF-κB p65 and IL-6. There is a positive correlation between the them(r=0.749,P<0.01).Conclusion: There was a positive correlation between the expression of immune adjusting factor IL-6 and NF-κB transcription factor in brain glioma(P<0.01), in the normal brain tissue was not expressed while in gliomas were expressed, and the higher degree of malignancy expression rate is obvious. So IL-6, NF-κB expression, occurrence and development in glioma are closely related, play an important role. |
