The Preparation Of Tetraphenylethene-Based Fluorescence Probe And The Improvement On Flow Cytometer

Traditional fluorescent materials will produce fluorescence quenching effect in the case of high concentration, seriously affecting its luminous effect. Aggregation-induced emission (AIE) phenomenon is just the opposite. With solute concentration and the degree of aggregation increased, luminous effect is getting obvious gradually. If the fluorescent probe is made of the new material with AIE effect, it will not only show a good luminous effect, but also have the advantage of easy storage and wide ranges.There are a lot of molecules which have AIE effect, tetraphenylethene and its derivatives play an important role and attract scientists’ much attention for its excellent light-emitting properties, simple synthetic route, and low cost.Our group used tetra-phenylethylene as the main base, designed and synthesized a series of tetra-phenylethylene-based compounds. All products have been proven by infrared spectroscopy, UV spectroscopy, and NMR analysis. The aggregation induced emission characteristic was tested, and so was the responsiveness to metal ions and DNA molecules. Explore whether the products can be used as the probes to detect metal ions and molecules of DNA molecules. New research applications in cell imaging and cytotoxic features were studied as well.Experimental results show that a series prepared tetraphenylethene compounds have good AIE effect. Among the products, DATPE and TATPE have good solubility to water. DATPE can be used as a fluorescent probe to detect Cu2+within a certain concentration range at a concentration of S×10-4mol/L. TATPE can be used as a fluorescent probe to detect Ag+within a certain concentration range at a concentration of 5×10-4mol/L. In the solution in which the concentration of DATPE is 10-5Mol/L, when the concentration of ctDNA raises from 0 to 70μM/mL, the luminous intensity increased nearly 11-fold. The experiments on chicken embryo fibroblasts, pancreatic stem cells and liver cells show that when DATPE is at a concentration of 0.5 mg/mL, cell imaging is pretty good, and it is available to be used as a cell imaging fluorescent dyes. When the concentration is controlled under 0.5 mg/mL, DATPB is totally no harm to the cells.Flow cytometry (FCM) is a kind of the new scientific instruments which is used as a core technology of flow cytometry sorting labeled cells. Flow cytometry can make the analysis and identification of the content and type of individual cell or several cells simply and quickly. At the same time, it also can tell out the cells containing even the specified chemical substance and separate different cells by the distinction in containing. That is why the flow cytometry is one of the most advanced equipment in the field of modern scientific research. Our research group also improved the original flow cytometry, flow cytometry was modified upon the original system, and a cell selecting channel was added, so that a flow cytometer can separate faster and get a higher purity separation, which effectively improve the separation efficiency.