Design,Synthesis And Biological Studies Off Luorescence Probe For Detection Of N₂H₄ And H₂S

In the sophisticated human body,most biochemical reactions and signaling pathway are involved in daily life activities.It can be achieved to control the physiological condition through balancing the biochemical molecular in the body.While the sorts of molecular,anions and metal ions play an important role in these reactions.It will threaten the human health,more seriously cause the death,if these molecular and ions are out of balance.We are eager to exploit a new technology to detect the small biochemical molecular and ions because the traditional skills,such as chromatography and electrochemical detection,are very complicated that it not only includes complex preparation methods and special equipment but also may destroy the structure of living cells.With the increasing development of optical technology,the detection of fluorescence probe for small biochemical molecular begin to spring up.It means the fluorescent dyes can react with the analyst and then we can observe the signal of fluorescence change through the advanced instrument of laser confocal fluorescence microscope.Finally it can be realized to trace the biochemical molecular in the level of cell or organelle.In consideration of the toxicity of N2H4 to the environment and human’s health and the limitation of fluorescence probe for the detection of N2H4 in living cells,we have developed a new novel colorimetric fluorescent probe for the detection of N2H4 in the second chapter.Moreover with high interest inH2S,the endogenously produced gaseous,the author designed and synthesised a new fluorescence probe for the detection of H2S based on the"ON-OFF" theory in the third chapter.The work are classified by the following two parts.1.The synthesis and biological study of A resorufin-based colorimetric and fluorescent probe for live-cell monitoring of hydrazineRosorufin is a kind of fluorophore goup with ideal properties because of its excellent photophysical and photochemical properties,such as pronounced colorimetric possibilities,good water-solubility and easy fluorescence quenching via 7-hydroxy substitution.We developed the fluorescence probe of N2H4 whose acronym is HFP.After reacting with N2H4 in the living cells,the HFP solution released the free resorufin with red fluorescence through the subsequent process of substitution,cyclization,elimination.This OFF-ON fluorescent probe HFP shows a red-shifted absorption spectrum and the color changes from colorless to red upon addition of hydrazine in the aqueous solution.Moreover,this probe also shows a significant fluorescence increase and excellent linear relationship at physiological pH.These results are successfully proved in CHO cells.There is a great increase of fluorescence in CHO cells with HFP after adding into the N2H4.HFP show the potential of monitoring the N2H4 in living cells.2.The synthesis and biological study of fluorescent probe for detection of hydrogen sulfide by a selective "on-off-on"H2S,as the gas signaling molecular,is the product of GSH and Cys.The innormal concentration of H2S will cause disease,such as the Alzheimer’s disease,Down’s syndrome.Taking into consideration that various fluorescence probes for H2S detection have developed with some limitations of serious condition for reaction with analytes.We decide to construct a new probe whose acronym is NJ1 for H2S detection based on the theory of the precipitation between Cu2+and H2S.Firstly,NJ1 can react with Cu2+,resulting in complex NJ1Cu formation,along with the fluorescence quenching.Then the NJ1Cu aqueous solution was described as the increase of fluorescence intensity with the addition of hydrogen sulfide.This "on-off-on" type fluorescence recognition system was a reversible process,which could be utilized to monitor copper ion and hydrogen sulfide based on a complex NJ1Cu formation-Cu2+displacement approach.Importantly,this real-time recognition process of Cu2+ and hydrogen sulfide exhibited excellent anti-interference ability.In addition,this new fluorescent probe also has potential utility for Cu2+and hydrogen sulfide detection in living cells,providing a potential tool for investigating copper ion and hydrogen sulfide in living systems or environment.