Study On Formaldehyde Fluorescent Probe Based On BODIPY Dye

Formaldehyde is a kind of carbonyl compounds with high activity,which distribures universally in various human organs as the metabolite intermediates.Under the catalysis oflysine-specific demethylase 1(LSD1)and semicarbazide-sensitive amine oxidase(SSAO),amino acids and exogenous substances are able to generate formaldehyde duringthe metabolic process.Formaldehyde can not only form stable methylene bridges with monoamines and phthalamines by interacting with them,but have impacts on DNA and proteins through some cross-link effects.It has been reported that the deflection of endogenous formaldehyde is related with series of disease,such as nerves system diseases,cardiovascular and cerebrovascular diseases and cancer.Thus,it has significant biomedical importance to detect formaldehyde in vivo.Compared with other biological detection modalities,fluorescence detection technology has attracted great attention due to several faverable characteristics,which include high sensitivity and selectivity,the real-time and in situ detection,and noninvasion on samples.Wedesigned and synthesized a BODIPY turn off fluorescent probe BoN for monitoring formaldehyde.With amino as the response group,the probe demonstrated high selectivity for formaldehyde in the presence of multiple active carbonyl species.We have also valited that LSD1 plays an important role in the genertion of formaldehyde.Besides,our probe has beensuccessfully applied to the detection of exogenous and endogenous formaldehyde in living cells and in vivo.The concentrations of formaldehyde in the homogenate from organs of mice(heart,liver,spleen,lung,kidney and brain)are also tested in our experiment.The paper is divided into four parts,as follows:1.The brief introduction of fluorescence imaging technology,BODIPY fluorophore and the design mechanism.A review of reported fluorescence probe for formaldehyde detecting in respects of design mechanisms,response mechanisms,and biological applications.Developing simple and sensitive fluorescent probes has great biological and medical significance for studying life activities.2.The introdution of the specific steps for the design and synthesis of the new type BODIPY fluorescent probe,and the mechanism for formaldehyde detecting.3.The detection of exogenous formaldehyde was achieved by using the new type BODIPY fluorescent probe.The spectra of the probes were tested using an ultraviolet spectrophotometer and a fluorescence spectrophotomete.Andselectivity of the probewas further investigated.4.The detection of formaldehyde in cells,tissues,and in vivo with our probe.Theassessment of the performance of our probe using a fluorescence spectrophotometer,laser-scanning microscope and in-vivo imaging system.The detection of the concentrations of formaldehyde in cells,tissues,and in vivo.And the validation of LSD1 enzyme plays an important role in the formation of formaldehyde.