Inunction Chinese Medicine Treatment Of Hemorrhoids Fibroblast Function Influence Mechanism Research

Objective:The aim of this study is to investigate the clinical application of tradition Chinese medicine in hemorrhoidal disease. The jiuhuagao was selected and applied by inunction treatment.The morphology of hemorrhoid and function and viability of fibroblasts cells were observed after the application of jiuhuagao. Our research provides the principal mechanism to the clinics.Methods:30 patients of hemorrhoid at the secondary stage were selected and jiuhuagao was applied as the only medicine in the process. The size of hemorrhoid, volume of blooding, anabrosis and prolapse were recorded and statistically analyzed. 31 patients were divided into three groups according to the pathological features and samples were taken during the surgery. Samples were then fixed by paraffin for histology observation. The change of the vessels and tissues were recorded after HE staining. In addition, TEM was applied to observe 1 sample of the hemorrhoid tissues after jiuhuagao application and 2 untreated samples.Results: According to the clinical experiments, jiuhuagao is useful to hemorrhoid. The values for the number of the prolapse of hemorrhoid, the size of hemorrhoid and the anabrosis of mucosa were 15.425, 11.195, and 10.033 before and after the treatment(P<0.05), respectively, indicating the significant differences for the treatment. While, no statistical significance were uncovered for the number of fibroblast cell, connective tissue、the degree of inflammatory cell infiltration、sinus veins dilation and vascular enendothelial proliferation(P>0.05). The survival rates of fibroblasts cells and arrangement of collagen fiber were all become normal after the application of jiuhuagao according to the optical microscope and TEM observation.Conclusion: The tradition Chinese medicine, jiuhuagao, is useful in hemorrhoid treatment. It can elevate the viability of the fibroblasts cells, which further influence the pathology morphology of collagen fibers, the arrangement of the collagen fiber and elastic fiber.