Effection Of Nux Vomica And Radix Dipsci On Cells Apoptosis And Signal Transduction Pathway Of Adjuvant Arthritis Rat

Objective: Experimental study on the effect of anti-inflammatory effect of nux vomica and Atractylodes macrocephala Koidz prior based on different ratio, This topic from the apoptosis and signal transduction pathway two, Further explore the mechanism of its anti inflammation and apoptosis, And to seek the best ratio, To provide a scientific basis for clinical rational application of nux vomica treating rheumatoid arthritis.Methods: 1.Wistar rats were randomly divided into blank group, model group, Nux vomica single decoctiongroup, Atractylodes macrocephala single decoction group, Nux vomica and Rhizoma Atractylodis Macrocephalae 1:12, 1:18, 1:24 Decoction group 7,Each group of ten only, male and female half and half. In addition to the blank group, the rats in the right rear paw pad intradermal injection of complete Freund’s adjuvant(CFA) 0.1ml, Copy the model of AA rats in different time, measured with a micrometer, left right pawthickness, To observe the effect of different compatibility of nux vomica and Baizhu group on AA in ratprimary, secondary paw swelling; Called the thymus, spleen weight, calculate the coefficient of immune organs;2.Using enzyme-linked immunosorbent assay(Elisa) determination of changes of serum cytokine levels in AA rats; 3. The expression of Western blot method for the determination of the synovial tissue Fas/FasL,Bcl-2/Bax, P53, Caspase3 protein, And explore the compatibility effects on the apoptosis pathway.Results: 1.In the reduction of primary AA rats toes swelling effect of Strychnos nux vomica Decoctiongroup and Rhizoma Atractylodis Macrocephalae 1:24; In the reduction of secondary AA rat paw swelling of nux vomica and Rhizoma Atractylodis Macrocephalae, 1:18 1:24 Decoction group is better.2. Nux vomica Decoction group And Rhizoma Atractylodis Macrocephalae 1:18, 1:24 has a protective effect on the spleen of AA rats.3. Nux vomica Decoction group and Rhizoma Atractylodis Macrocephalae 1:24 could significantly reduce TNF- alpha content in serum of rats.4. The compatibility of semen Strychni hematoxylin 1:18, 1:24 Decoction group can express thesynovial tissue was up-regulated in Fas; Expression of 1:12, 1:24 and Rhizoma Atractylodis Macrocephalae nux vomica Decoctiongroup can significantly inhibit the apoptosis of factor FasL in synovium tissue; Expression of nux vomica and Rhizoma Atractylodis Macrocephalae 1:18 Decoctionsignificantly inhibited Bcl-2 cell apoptosis factor in synovial tissue in AA rats; Nux vomica and Rhizoma Atractylodis Macrocephalae 1:18 Decoction groupexpressed significantly up-regulated the expression of AA in rat synovial Bax; Nux vomica And Rhizoma Atractylodis Macrocephalae1:18 Decoction group can significantlyinhibit the expression of P53 synovial tissue of AA rats; The compatibility of nux vomica Decoction group 1:18 could express Caspase3 synovial tissuesignificantly upregulated AA in rats.