| Objective:The experimental animal models used in this study copied foodborne obese rats, which were therapied by EA. To observe experimental indicators of each group of rats after treatment,including the expression of skeletal muscle CPT-1 and PGC-1α, and how to improve insulin resistance in obese rats, to analyze and discuss the cellular and molecular mechanisms.Methods:Manufacturing foodborne obese rats:40 SD rats were randomly divided into two groups, normal control group (8) fed by normal diet and high-fat group (32) fed by high fat diet. After 12 weeks, the body weight of high-fat group exceed 20% to the control group to determine foodborne obese rats. Finally, the success rate of modelling obese rats is 51.2%, a total of 16. The successful modelling obese rats were randomly divided into two groups:EA group (6) and the model group (6). EA group:acupunctured unilaterally at "tsusanli" or "chambers", roated the acupunture point every other day, and connected Han electro-acupuncture device each time, strength 2mA, frequency 2/15Hz, time 15min; model Group:bundled only 15 minutes, the same way as acupuncture group; normal control group:did not do therapy or bondage. Six days for treatment each week,one day for rest, The courses is 4 weeks. During treatment three groups of rats were fed with normal diet. Compared body weight and food intake of each experimental groups of rats before and after treatment,compared the body fat, total cholesterol, triglycerides, glucose after treatment.Measured insulin by ELISA, observed liver pathological changes by HE,and detected the rat skeletal muscle CPT-1α, PGC-1α mRNA expression by real-time PCR.Results:l.The effects of body weight in obese rats:before treatment of EA,body weight in obese rats of model group and EA group were compared with and normal group,the differences were significant (P<0.01); the mean weight of two groups of obese rats was no significant difference (P> 0.05); there was statistically significant difference between EA group and model group in rats body weight(P<0.05) after EA treatment, and there was no statistically significant difference between EA group and normal group in rats body weight (P> 0.05).2. After the end of the treatment the difference in viscera fat of EA group and model group was significant (P<0.01),compared with EA group and normal group, there was no significant difference (P> 0.05); after treatment, the difference in brown fat of EA group and model group was statistically significant (P<0.05),and there was no significant difference in EA group and normal group (P> 0.05);compared with model group,body fat ratio in neck of EA group had no significant difference (P> 0.05),compared with model group,there was no statistically significant difference (P<0.05), compared with the normal group, the difference was not significant (P> 0.05), indicated that EA therapy could improve the body fat in obese rats.3.EA therapy could obviously improved serum lipids in obese rats:after treatment,the difference of serum total cholesterol of EA group and model group was significant (P<0.01),and there was no significant difference between EA group and normal group (P> 0.05); the difference in serum triglyceride in EA group and the model group was significant(P<0.01), and there was no significant difference between EA group and normal group (P> 0.05).4. Effect of EA on insulin levels in obese rats:compared with model group,the differences in insulin content of EA group and the normal group EA group were statistically significant (P <0.01, P<0.01),but there was no tatistically significant difference between EA group and normal group. (P> 0.05).5.EA therapy significantly improves for elevated blood glucose in obese rats:To compare with the normal group and EA group, the differences in blood glucose of model group were statistically significant (P<0.01); there was no statistically significant difference between EA group and normal group (P> 0.05).6.EA therapy could obviously improve mRNA expression of CPT-1 and PGC-1α in obese rats:compared with normal group,levels of the mRNA expression of CPT-1 and PGC-1αin the EA group were both induced after treatment, compared with model group,the mRNA expression of CPT-1 and PGC-1αin skeletal muscle of EA group muscle were both increased.7. Effect of EA on bone morphogenetic of obese rats:obese rats skeletal muscle lipid deposited, lipid droplets increased, capillary density decreased, muscle fiber cross-section extracellular matrix reduced; The lipid depositions of rats in EA group are improved, lipid droplets in muscle fibrils are reduced, heterogeneous staining are decreased.Conclusion:EA therapy could reduce body fat, blood lipids and blood sugar of obese rats,inhibit fat synthesis and (or) promote lipolysis. It enabled increase the expression of mRNA of CPT-1 and PGC-lain skeletal muscle of obese rats, increase insulin levels,and enhance insulin sensitivity to improve hyperinsulinemia insulin resistance. |
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