TCT, HPV Detection And Cervical Disease Correlation Studies

Objective: To investigate(1)The human papilloma virus(Human Papilloma Virus, HPV) infection subtype distribution in the region, a high incidence of age, as well as high-risk HPV infection and HPV infection;(2)Different ages Thinprep Cytology Test(TCT) the distribution of test results;(3)TCT, HPV, and the value of screening for cervical lesions in both the joint detection.Method: Collected from January 2012 to December 2014 for three years in Wannan Medical College Hospital Yijishan, HPV type detection of 14386 cases; TCT of 40933cases; cervical tissue pathological examination combined TCT of 1749 cases; joint HPV examination of 1636 cases; and TCT joint of HPV and cervical tieeue examination resuits of 859 patients, knowledge of the TCT, HPVand cervical disease.Results: 1、TCT joint cervical biopsy of 1749 cases, TCT examination for benign lesions 1095, ASCUS of 270 cases, LSIL of 108 cases, HSIL of 276 cases. The sensitivity of the TCT examination was 65.50%, false negative rate was 34.5%, positive predictice value was 88.53%, negative predictive value of 72.15%. TCT examination results compared with cervical histopathological findings, CIN Ⅰ group and CIN Ⅱ compared difference was statistically significant,(χ2= 6.008, P﹤0.05), CIN Ⅱ group and CIN Ⅲ compared difference was statistically significant,(χ2= 13.819, P﹤0.05), CIN Ⅲ group there was no statistically significant difference compared with cervical cancer,(χ2= 1.037, P﹥0.05).2、40933 cases of cervical TCT examination results show that: TCT results for normal or inflammation, the maximum percentage of 62.61%, followed by HSIL was 15.78%, 15.44% of ASCUS, LSIL is 6.17%. ASCUS in 40 ~ 44, 45 ~ 49 age group detection rate is higher, LSIL, HSIL also in 40-44, 45- 49 years old age group high detection rate.3、Total inspection type HPV-DNA testing samples of 14385 cases of positive cases of 2833 cases, HPV infection rate was: 19.69%(2833/14386); High-risk type HPV2260 example, high-risk type HPV infection rate was15.71%(2260/14386); Low-risk type HPV242 example, low-risk HPV infection rate is: 1.68(242/14386). One of the most common HPV subtypes in the order(from high to low) : 16(3.27%), 51(1.97%), 56(1.61%), 58(1.54%), 31(1.28). With high risk, low risk, Chinese common subtype infection rates were 15.71%, 1.68%, 2.30%, high-risk pattern compared with low-risk type difference was statistically significant(P﹤0.05). High-risk type HPV infection in the distribution of ages: ﹤24(13.78%), 25 to 29(13.84%), 30-34(%), 35 to 39(17.84%), 40 to 44(19.94%), 45(19.90%), 49, 50-54(24.08%), 55 and 59(27.49%) and 60 years old(21.83%), or high-risk type HPV infection incidence age between 40 to 59 years old.4 、 Joint histologic examination of 1636 cases of cervical HPV, cervicitis in HPV infection rate was 36.38%, high-risk type HPV infection rate was 35.17%; HPV infection rate was 64.81% in CIN Ⅰ level, high-risk type HPV infection rate was 61.11%; HPV infection rate was 72.38% in CIN Ⅱ-Ⅲ, high-risk type HPV infection rate was 70.63%; Cervical HPV infection rate was 93.20%, in high-risk type HPV infection rate was 92.23%. In cervicitis group as the main subtype infection, 106 cases(33.23%), followed by: 52, 18, 58, 31; CIN Ⅰ level in the group as the main subtype infection, 10 cases(30.30%), followed by: 52, 33, 45. With cervicitis in CIN Ⅱ group as the main subtype infection, 106 cases(33.23%), followed by: 52, 18, 58, 31; CIN Ⅲ HPV16 also as the main subtype infection, 127 cases(45.03%), followed by: 59, 31, 56, 58. HPV16 infection in cervical cancer in 77 patients(81.05), followed by, 18, 33, 56, 58.5、HPV infection in cervical histopathological examination sensitivity was 74.76%, 75.82%, positive predictive value was 62.28%, negative predictive value was 75.82%, the false positive rate was 24.18%. HPV infection rate was in cervicitis: 36.38%, CIN Ⅰ for: 64.18%, CIN Ⅱ for: 72.25%, CIN Ⅲ for: 72.43%, 93.20% in cervical cancer. CIN Ⅱ- Ⅲ group difference was statistically significant compared with cervical cancer group,(P﹤0.05).6、HPV, TCT joint inspection check one or two positive for 859 cases, the sensitive rate was 89.00%. TCT associated with the comparison of HPV detection, TCT joint HPV detection and a detection method of comparison, the difference had statistical significance(P﹤0.05).Conclusion: The region’s women subtypes distribution of HPV infection has obvious difference and age distribution heterogeneity, women at high risk type HPV infection rate increased with the growth of the age, tip in clinic in the future we should pay attention to high-risk subtypes of HPV in the region and high-risk age, be timely, targeted early screening of cervical disease; And in the future use of HPV prophylactic vaccine at the same time considering the local factors to distinguish the type and age.