Study On Differences Of Atherosclerotic Plaques Between Different Drinking Patterns Of Rat Atherosclerosis Models Which Were Set Up By Calcium Overload

Objective: To investigate the differences of atherosclerotic plaques between different drinking patterns of rat atherosclerosis models which were set up by calcium overload.Methods: 80 adult SD rats were divided into 4 groups, respectively were Control group, model control group (Model group), freely drink group (F-D Model group) and intermittent gavage group(G Model group), and each of them included 20 rats. During the first 3 weeks, rats in Model group, F-D Model group and G Model group were intraperitoneal injected with Vitamin D3 and fed on high lipid feed, while rats in Control group were intraperitoneal injected with same volume of normal saline (NS) and fed on normal feed. From the 4th week, we replaced the drinking water of F-D Model group with alcoholic beverages containing 6% alcohol so that the rats in this group could drink freely. In the 6th,8th,10th,12th,14th and 16th weeks, rats in all groups were fasted for a night and then given by gavage every 2 minutes. Rats in G Model group were given 50% alcohol with a dosage of lg/kg each time, the total dosage of which is 7g/kg, while rats in other groups were given the same volume of NS. Finally, at the end of 18th week, all the rats were anaesthetized and drawed blood from abdominal aortas to assay biochemical criteria such as TG, HDL-C and LDL-C. At the same time, tissues of their aorta arches and livers were taken down for HE staining, then the pathologic changes of which were observed.Results:(1) Pathological Results:Condition of aorta arches:Indexes of Control group rats were as follows:endangium structures were integrity, vascular walls were free of incrassation, medial smooth muscles were arranged orderly and serried, leading to distinct gradations. Indexes of Model group rats were as follows: endangiums were incrassated, medial smooth muscles were arranged disorderly and incompact, leading to indistinct gradations, with calcified plaques and cholesterol crystal. Indexes of F-D Model group rats were as follows: endangium structures were broken, vascular walls were severe incrassated and calcified, fibrous caps were ruptured and large lipid drops within it were shown, medial smooth muscles were arranged disorderly and incompact, with large areas of calcification and several fractures, leading to irregular shapes. Indexes of G Model group rats were as follows:endangiums were incrassated, medial smooth muscles were arranged mainly orderly but incompact, leading to distinct gradations, with partial calcifications and bits of cholesterol crystals. Condition of livers:Indexes of Control group rats were as follows:Organization structures of livers were distinct, central veins were arranged radially around hepatic cords, shapes of liver cells were normal. All of these indexes were similar to Model group, F-D Model group and G Model group.(2) Serological detection: TG level of Model group, F-D Model group and G Model group were higher than which of Control group (P<0.05). TG level of F-D Model group had no significant difference with Model group (P>0.05), but which of G Model group was lower than other groups (P<0.05). HDL-C level of G Model group was slightly higher than which of Control group and Model group (P<0.05). There was no significant difference between Model group and F-D Model group (P>0.05), and no significant difference between G Model group and F-D Model group (P>0.05) as well. Control group had the lowest LDL-C level (P<0.05), while G Model group had the lowest LDL-C level (P<0.05)in all model group, and there was no significant difference between Model group and F-D Model group (P>0.05).Conclusions:1. Low dose of continuous drinking can aggravate arteriosclerosis of rat atherosclerosis model set up by calcium overload; High dose of intermittent drinking can prevent rat atherosclerosis models set up by calcium overload from arteriosclerosis to some extent.2. High dose of intermittent drinking leads to lower TG level of rat atherosclerosis models set up by calcium overload, while low dose of continuous drinking hasn’t obvious effect on it.3. High dose of intermittent drinking leads to higher. HDL-C level of rat atherosclerosis models set up by calcium overload, while low dose of continuous drinking hasn’t obvious effect on it.4. High dose of intermittent drinking leads to lower LDL-C level of rat atherosclerosis models set up by calcium overload, while low dose of continuous drinking hasn’t obvious effect on it.