Expression And Significance Of RUNX3 In Actinic Keratosis And Cutaneous Squamous Cell

[Backgrand]RUNX3 as a cancer suppressor gene has been first studied in gastric cancer,then has confirmed it is a cancer suppressor gene in the colorectal cancer,lung cancer,keloid.RUNX3 protein is a growth factor beta(transforming growth factor beta.TGF-beta) signal transduction pathways downstream of a transcription factors and TGF-beta is negative regulation of epithelial cell growth factor,mediating cell proliferation and differentiation,angnogenesis,tumor and other biological events,the signal transduction pathway disorders can lead to a variely of tumors.In recent years,some scholars put forward the opposite opinion,saho studies have shown that such as TeHez M RUNX3 protein expression in basal cell carcinoma is excessive,so RUNX3 tumorigenic or tumor suppressor genes in tumors need to know and study.[Objective]1.To investigate the expression of RUNX3 in Actinic Keratosis and Squamous Cell Carcinoma. Analysised the clinical signification and mode of action of RUNX3 in Actinic Keratosis and Squamous Cell Carcinoma.2.RUNX3 that maybe as the important biological indicators in the dermatoma diagnosis and differential diagnosis and the malignant grades.[Methods] The patients who diagnosed pathologically in dermatology department and pathology department accord with the diagnosis standard as Actinic Keratosis 30 cases. Bowen Disease 30 cases, Squamous Cell Carcinoma 30 cases, meanwhile, we also collected normal skin 10 cases from operating room of our department and dent facial surgery. After steps of dehydration, embedding, slicing and staining with immunohistochemistry (SP Method), observing slices under the microscope, each slice in the skin diseased tissue randomly selected five discontinuous visions; take a picture with OLYMPUS BX51 Microscopic Image Acquisition System. Quantitative determination of each field of vision of positive expression of the average optical density with Image-Pro Plus 6.0 (IPP) Image analysis software.[Results]1.RUNX3 in 10 cases of normal skin,30 cases of Actinic Keratosis,30 cases of Bowen Disease,90 cases of well, moderately and poorly differentiated squamous cell carcinoma of the skin in all have positive expression. RUNX3 in the expression of the average optical density between categories is different or not all the same (P< 0.05), the difference was statistically significant.2.Normal skin, RNUX3 is mainly expressed in epidermal basal layer cells and basal layer cells of the cytoplasm, the dye from basal layer to surface density and intensity gradually weakened, around the hair follicles, sweat glands and sebaceous glands of basaloid cells visibleto positive expression;the cytopiasm is tan particles,in fat cells and mesenchymal tisuue cells without expression. Actinic Keratosis, RUNX3 mainly expressed in epidermal basal layer and stratum spinosum lower 1/3 in the cell cytoplasm, base layser is brown granules in the cell cytoplasm stratum spinosum cell cytoplasm is tan particles,dyeing density and strength, in the middle of stratum spinosutom positive expression is scattered, the dermal papilla layer around a few positive lymphocytes expression; Bowen Disease, RUNX3 is mainly expressed in skin full-thickness atypical hyperplasia cutin cell cytoplasm formation,the cytopiasm is tan particles, the dermal papilla layer around a few positive lymphocytes expression,Well differentiated squamous cell carcinoma, RUNX3 main expression is diffuse part around the nests of basaloid squamous cell cytoplasm of cells and nest, the cytopiasm is tan particles,around the nests around a few positive lymphocytes expression; Moderately differentiated squamous cell carcinoma, RUNX3 mainly is diffuse expression in squamous cell carcinoma of the nest, the cytopiasm is tan particles, around the nests around a few positive lymphocytes expression;Poorly differentiated squamous cell carcinoma, RUNX3 mainly diffuse expression in squamous cell carcinoma in the nest, the cytopiasm is tan particles,around the nests around a few positive lymphocytes expression.3.By IPP software statistics, RUNX3 expression as follows:the average optical density in normal skin is 0.1841±0.0022; the average optical density in Actinic Keratosis is0.1922±0.0025;the average optical density in Bowen Disease is 0.1926±0.0019; the average optical density in well differentiated Squamous Cell Carcinoma is 0.1943±0.0025; the average optical density in moderately differentiated Squamous Cell Carcinoma is 0.1945±0.0028, the average optical density in poorly differentiated Squamous Cell Carcinoma is 0.1946±0.0033. Among them, the RUNX3 strongest in the expression of poorly differentiated squamous cell carcinoma group, second is moderately differentiated squamous cell carcinoma group respectively, well squamous differentiated cell carcinoma group, Bowen Disease group, the Actinic Keratosis group and normal skin group.4.Statistical analysis, RUNX3 in the expression of the average optical density between categories is different or not all the same (P< 0.05), the difference was statistically significant. Further pairwise comparison, the normal skin group and Actinic Keratosis group, the normal skin group and Bowen Disease group, the normal skin group and well differentiated squamous cell carcinoma of the skin, normal skin group and moderately differentiated squamous cell carcinoma group, normal skin group and poorly differentiated squamous cell carcinoma of the skin, Bowen Disease group and well differentiated squamous cell carcinoma group, Bowen Disease group and moderately differentiated squamous cell carcinoma group, Bowen Disease group and poorly differentiated squamous cell carcinoma, P is less than 0.05, differences were statistically significant. The rest two comparison between groups (P > 0.05), there were no statistically significant difference.5. RUNX3 and expressed in each of the groups in the patients with different gender, different age, area of exposure and the exposure area were no statistical difference (P > 0.05). [Conclusion] 1.RUNX3 in normal skin, Actinic Keratosis, Bowen Disease, well, moderately and poorly differentiated squamous cell carcinoma of the skin in all have positive expression.RUNX3 has played a certain role in the development of skin squamous carcinoma. 2.RUNX3 expressed showed a trend of rising in normal skin,precancerous tissue and squamous cell carcinoma.RUNX3 may play a role in epithelial tumor carcinogenesis. 3.The positive expression rate of RUNX3 is higher; RUNX3 can be as a important basis in skin squamous carcinoma early and differential diagnosis.It provided a new way for tumor treatment and prognosis.4. Positive expression rate of RUNX3 with different gender, different age, area of exposure and the exposure area were no statistical differences.however,it associated with tisuue differentiation degree. RUNX3 that maybe as the important biological indicators in the derrnatoma diagnosis and differential diagnosis and the malignant grades.