Assocciation Of Osteocalcin With Obesity,sugar Metabolism,and Lipid Metabolism In Children

Objective : Obesity is a complex process, many factors are involved in process.It seriously affect the metabolism of sugar and fat. More and more studies have shown that the changes of endocrine hormone level can affect the incidence and development of obesity, at the same time, participate in the metabolism of sugar and fat. Exploring the reason and mechanism of childhood obesity, finding the prevention and treatment of childhood obesity, disorders of sugar and lipid metabolic can reduce the occurrence of adult obesity and metabolic syndrome.Recent studies have reported the specific hormones- osteocalcin, has a potential relevance with fat and the sugarl and lipid metabolism.Many animal and clinical experiments showed that osteocalcin can adjust fat content, inhibit insulin resistance,lower blood sugar, increase insulin secretion.And in the animal experiments, the obese mice are injected subcutaneously osteocalcin can obviously reduce weight, treat obesity.In this paper, through a case-control study,analysing the relationship between the fat, sugar, lipid metabolism and osteocalcin,by collecting physical measurement,blood glucose, insulin, blood lipid from obese children and a random sample of non-bese children nearly 2 years in our hospitalMethods:Choosing 66 obese children and 40 non-obese children randomly selected the same period in March 2013- January 2015 clinic in our hospital pediatric were retrospectively analyzed. There were no high blood pressure, diabetes, cardiovascular disease in 2 group, recently not bone metabolism drugs,also with the exception of pathological obesity caused by other diseases.1.According to growth curve of body mass index in the Chinese children from 0 ~ 18 years old is divided into obesity group and the non-obesity group, analyzing the detection index and the factors influencing the osteocalcin in two groups.2.According to quartiles of BMI, 66 obese children are divided into A1, A2, A3, A4, 40 non-obese children are divided into A0,analyzing the differences and changes trend of each group.3.According to the quartiles of osteocalcin,106 children are divided into B1, B2, B3, B4, analyzing the differences and changes trend of each group.The next morning after admission and, after micturition and taking off the coat and shoes and hats,measured height, weight, at the same time,empty stomach> 8 hours( no eating any food, drinking water), extraction of venous blood 8 ml before breakfast, automatic biochemistry analyzer in the same period were used to detect fasting blood glucose(FBG), triglyceride(TG), low density lipoprotein(LDL),high-density lipoprotein(HDL), alkaline phosphatase(ALP).And return of blood samples, 2 divide separating serum, storage in- 20 ℃ and-80 ℃ refrigerator, put to use insulin free kit and electrochemical luminescence osteocalcin detection kit(the first affiliated hospital of dalian medical university from center, nuclear medicine chamber test) test fasting insulin and serum osteocalcin. Using the steady state model(HOMA IR)and insulin resistance index calculated islet beta cells secrete index(HOMA- beta).Using SPSS19.0 statistical software to analyze the clinical data, P < 0.05 for the difference was statistically significant.Results:1.In 66 cases of obesity group, children’s BMI, TG, LDL, FINS, HOMA IR,HOMA-β levels are compared with 40 cases of non-obesity group is high, the low level of HDL, OC, the difference is statistically significant(P < 0.05). But in the comparison of SBP, FBG, there is no statistically significant difference(P > 0.05).2. In 66 obese children, according to the bivariate analysis, OC and BMI, FBG, TG,LDL, FINS, HOMA- IR are negatively correlated(P < 0.05), and HDL are positively correlated(P < 0.05). In the 40 children non-obesity group, according to the bivariate analysis, OC content is positively correlated with Age, HOMA- IR(P < 0.05), and is negatively correlated with BMI and SBP(P < 0.05).3.In 106 cases of children, obese children are divided according to BMI quartiles,There is the difference between groups in BMI, FBG, TG, LDL, HDL, FINS, OC,HOMA IR, HOMA- β(P < 0.05), the Age, Sex, SBP,ALP has no statistical difference between groups(P > 0.05).4.In l06 children, according to the serum osteocalcin quartile groupings, There is the the difference between groups in OC, Age, BMI, SBP, FBG, TG, LDL, HDL, FINS,HOMA IR, HOMA- β(P < 0.05), and no statistical difference between the ALP in groups(P > 0.05). In 106 cases of children, by the bivariate analysis, serum OC content is positively correlated with Age(P < 0.05), and BMI, FBG, TG, LDL, HDL, FINS, and is negatively correlated with HOMA-IR, HOMA-β(P < 0.05).Conclusions:1. Serum osteocalcin level is affected by dietary factors.2.Osteocalcin regulates fat, reduces weight.3.Osteocalcin can promote insulin secretion, insulin resistance, maintain the stability of blood sugar,can reduce triglyceride and maintain cholesterol levels.4.Osteocalcin can be used as a monitoring index prediction obese children glycolipids change occurs, in 3 to 10 years old age children, when the OC < 46.52±1.64,prompt the emergence of sugar, lipid metabolic disorder.