Effects Of Phellodendron Leaves Extracts And Phellamurin On HepG2Cell

PLF is a kind of flavonoids extracted from the leaves of natural plantPhellodendron amurense. Phellamurin is a compound with single structure purified byliquid phase separation from PLF. In this paper, we mainly studied about the effects ofPLF and phellamurin on apoptosis, cell cycle and capacity of scavenging ROS inHepG2cells. The experiment results are as follows:Firstly, the capability of scavenge ROS of PLF and phellamurin in HepG2cellswere measured with flow cytometry. After800ug/mL phellamurin was added toHepG2cells for6,12,24hours respectively, the DCFH-DA probe were introducedinto cells and the level of ROS was measured by the intensity of intracellularfluorescence with flow cytometry. Compared with the control group, the level ofintracellular ROS in HepG2cells was only56.46%and53.9%after treatment with75ug/mLPLF and800ug/ml phellamurin, respectivaly, which showed their capacity ofscavenging ROS.Secondly, effects of PLF and phellamurin on HepG2cells’ apoptosis were alsomeasured with flow cytometry. The results showed that after treatment of drugs indifferent concentrations for48hours, HepG2cell proliferation were inhibited in adose-dependent manner. When the concentrations of PLF and phellamurin were1.5mg/mL, their inhibitory rate of HepG2cells were90%and70%, respectivaly.Finally, the same methods were used on effects of PLF and phellamurin on cellcycle of HepG2cells. The results indicated that, after incubation with1mg/ml drugsfor48hours, PLF induced a G1arrest in92%of HepG2cells. while phellamurinmade57%of HepG2undergo S cell cycle arrest.It is suggested that PLF has the capacity of inhibiting DNA topoisomerase andpreventing DNA from replicating in our previous studies. Our studies shows thatHepG2cells undergo G1cell cycle arrest, which is consistent with our previous studies and indicates the molecular mechanism of PLF inhibiting tumor proliferation.Our research indicates that PLF and phellamurin have the capacity of scavenge ROSand inducing HepG2cells apoptosis. Phellamurin can induce a S cycle arrest inHepG2cells, which may relate to CDK2and related study is under way.