Study On Anxiolytic Effects Of The Extracts From Cortex Albizziae By Screening And The Influence Of Neurotransmitters In The Brain

With the development of human’s lifestyle, mental illness was gradually occuping one of the leaders. Along with the development of science and technology, "anxiety" has become increa-singly popular.According to the difference of symptoms and on set time, anxiety also have different kinds. Anxiety can be divided into: generalized anxiety disorder,panic disorder,social anxiety disorder, post traumatic stress disorder and obsessive compulsive disorder.At present,the commonly used drugs in clinical anti-anxiety are: benzodia zepine drugs(BZD), non-benzodiazepine drugs and antidepressant drugs with anxiolytic effects.All of those drugshave different side effects. The new anti-a nxiety drugs such asZellers PL with a higher price, there are still side effects. Therefore, we have to look for an economic, litter side effect,wider use of anti-anxiety drugs.The Chinese medicine is cheaper, fewer side effects, etc. so that it has a clear advantage and application prospects in the treatment of anxiety d isorders. As our traditional sedative drugs,Cortex Albizziae,it been proved to h ave sedative effects by traditional clinical evidence of their sedative effect. Ina ddition,modern pharmacological research also shows that it has a strong anti-a nxiety effect. Ourteam has systematically studied preliminary extraction of Albzzia julibrissin water extraction method and purification process,We also establishde the quality evaluation system.We get six parts after the separation and purification:the three parts eluting from the D101 macroporous resin fractions(the water part,the 40% ethanol part and the 95%ethanol part)and the three components coming from the 40% ethanol part eluted after SKP RP resin(the 40% methanol part,the 75% methanol part and the 90% methanol part).In this paper, we investigated the anxiolytic effects of these six on the basis of animal behavior,such as, the elevated plus maze, light-dark test, open field test and a series of animal behavior experiments.We found the anxiolytic activity strong three parts,D-H20,D-40,S-75.UFLC-QTOF technology was used for vitro research Albizzia julibrissin fingerprints.The two parts(D-40 and S-75) enriched the water-Soluble ligna n. Lignan compounds may be one of its anxiolytic active substances.The method of simultaneous determination of seven kinds of different ne urotransmitters from,Cortex Albizziae anxiolytic animal brain tissue was estab lished by using UFLC-MS. This method has the advantage of high sensitivity, short analysis time.Also, it is more economical, efficient and sensitive than the traditional electrochemical method and enzyme-link immunoassay kit.After study on Methodology,the high variety of neurotransmitters in brain tissue, me dium and low samples’ day precision(RSD) values is 1.0%-4.6%, the value of inter-day precision RSD value is 2.0%-6.8%, accuracy value is 98.6%-104.2%, matrix effects value is 86.7%-95.7%, extraction recovery value is 81.5%-95.3%. All of this are in line with the biological sample testing requirements, t he results are accurate and reliable. Through the detection of anti anxiety in a nimal brain tissue amino acids, monoamine neurotransmitter, acetylcholine, Pre- liminary exploration of neural anxiolytic activity of Cortex Albizziae neurotransmitters, the results show that the anti- anxiety activity and elevated gamma aminobutyric acid(GABA),glutamate(Glu),acetylcholine(Ach),neurotransmitters and reduced serotonin(5-HT),5-hydroxyindole acetic acid(5-HIA A),norepinephrine(NE) and related neurotransmitters.The effect of decreased monoamine neurotransmitter content is more significant.It can lay the found- ation for later study of the anti-anxiety mechanism for later Cortex Albizziae.