| Objective: In this study, the detection of inflammatory cytokines IL-6, TNF-a unilateral cut in STZ-induced diabetic nephropathy rat kidney tissue expression and pathological changes in the structure of renal tissue, kidney investigate the role of diabetic nephropathy mechanism for this party to provide the basis for clinical application.Methods: 60 healthy male Wsitar rats were randomly divided into normal control group, 10, made 50 modules. Control group underwent sham left kidney removal surgery, making the module row left kidney removal surgery, two weeks of fasting intraperitoneal injection of streptozotocin(STZ) solution 40 mg / kg, 72 hours after the blood test blood glucose ≥ tail 16.7mol / L, urine ≥3 + last three days were successful model for inclusion in this experiment. Modeling fails to remove the rats, and then press the blood sugar levels were randomly divided into the model group, model group, large and small dose groups, each group Lotensin 10, administered eight weeks. The first weekend I were observed in rats 4,8 24-hour urinary albumin(UMA), serum creatinine(Scr), blood urea nitrogen(BUN) changes in renal pathological changes were observed by light microscopy, immunohistochemical assay each renal tissue IL-6, TNF-a positive expression.Compared with normal control group, model control group, high-dose, low-dose, Lotensin group of rats modeling success will appear more food, drink, urine output increased significantly, sparse hair dark color, weight loss and other symptoms, glucose significantly increased; but these symptoms performance model group gradually increased, along with the other three groups to extend the delivery time will be gradually reduced, as compared with the control group, these symptoms improved significantly decreased blood glucose, body weight gradually increased. 2 Comparison of two groups of rats UMA, Scr, BUN levels 4 weeks of treatment, the first 8 weeks, compared with the normal control group, the rest of the blood glucose rats were significantly higher, rising UMA, Scr, BUN levels, and the difference was statistically significant(P <0.01 or P <0.05); compared with the model group, drug treatment blood glucose rats in each group were slightly lower, UMA, Scr, BUN levels decreased, the difference was statistically significant(P <0.05); however, the first 4 weeks of drug treatment comparison between groups, UMA, Scr, BUN levels were not significant differences were not statistically significant. The first 8 weeks, compared with the high-dose group, low-dose group and Lotensin group UMA, Scr, BUN levels were significantly higher than high-dose group, the difference was statistically significant(P <0.05); low-dose group and Lotensin group compared, UMA, Scr, no significant difference in BUN levels, no significant difference(P> 0.05). 3 renal pathological morphology After 4 and 8 weeks after therapy, compared with the control group, the lower dose group, low-dose group, Lotensin glomerular congestion and edema, reduce the degree of proliferation of mesangial cells and extracellular matrix significantly reduced renal tubular epithelial cell swelling reduced, few tubular epithelial cell degeneration and inflammatory cell infiltration significantly improved; compared with the low-dose group, high dose group of glomerular, tubular pathology significantly improved; and Lotensin group, glomerular, tubular pathological changes in high dose group was significantly improved. 4 IL-6 expression by immunohistochemical staining of renal tissue, TNF-a in Normal renal tissue there is still a small amount of brown particles, a small amount of expression; compared with normal control group, model control group of rats with extended duration of expression gradually increased, and the difference was statistically significant(P <0.01); Compared with the model group, drug treatment groups in the reduction in renal tissue expression, and the difference was statistically significant(P The result: 1 A general<0.05); the first 4 weeks, each group therapy in renal tissue of rats considerable number of brown particles, the difference There was no significant difference(P> 0.05). The first eight weeks of treatment, compared with the low-dose group and Lotensin group, the expression of high-dose group was significantly less than in renal tissue small dose group, the difference was statistically significant(P <0.05); low-dose group and Lotensin group comparison, the number of kidney tissue quite brown particles, no statistical significance(P> 0.05).In conclusion: Yishentang can significantly reduce the inflammatory cytokines IL-6, TNF-a expression in renal tissue of diabetic nephropathy, clarified yishentang by reducing inflammatory cytokines IL-6, TNF-a content in the kidney tissue DN rats improve their inflammatory state, to protect the kidneys. |
PDF Full Text Request