Experimental Research Of EA Stimulation On Jejunum Movement At Different Intensities Mediated By Beta-adrenergic Receptor

Objective:Lots of clinical data showed that acupuncture can treat FGIDs, including functional dyspepsia, functional constipation, irritable bowel syndrome, etc. It is suggested that autonomic nerve is the main neuropathway in which acupuncture modulating gastrointestinal motility, and different intensities generate different effects through different peripheric afferent pathways. By comparing the difference of jejunal pressure of β receptor knockout mice and wild type(WT) mice stimulated by electroacupuncture at different acupoints, we investigate the relationship between jejunum motility stimulated by EA and sympathetic nerve system, especially the role of P receptor in EA stimulation on jejunum motility, and its possible afferent pathways.Methods:Animals were divided into four groups:CL57BL/6 WT mice, β1β2 receptor knockout mice, β1 receptor knockout mice and β2 receptor knockout mice,40 mice for each group, male. Then we divided each group into 5 subgroups with EA stimulation of 1mA,2mA, 4mA,6mA and 8mA in 2/15HZ. We Use self-made water bursa intraintestinal piezometric head monitoring intestinal pressure and observe the amplitude and frequency of jejunum motion. When the intestinal pressure is stable, we stimulate Shangjuxu, Quchi, Tianshu, Dachangshu with EA at the above intensities for 1 minute. The amplitude and frequency of jejunal motility before and during EA stimulation were recorded and analyzed.Results:1.EA stimulation at Quchi and Shangjuxu at 2mA,4mA,6mA,and 8mA on WT mice, increased the jejunal pressure significantly, while at Tianshu and Dachangshu with the same intensities, the pressure reduced significantly(P<0.05 or P<0.01),suggesting an intensities-effects relationship between the intensities and the pressure, and when the intensity reaches 4mA,the effect reaches a platform stage.2. EA stimulation at Quchi, Shangjuxu,Tianshu, Dachangshu at 4mA,6mA and 8mA on β1-/-β2-/-mice, increased the jejunal pressure significantly(P<0.05 or P<0.01),suggesting an intensities-effects relationship between the intensities and the pressure at all four pressurepoints, and when the intensity reaches 6mA.the effect reaches a platform stage.3. EA stimulation at Quchi, Shangjuxu at 2mA,4mA,6mA and 8mA on β1-/-mice, increased the jejunal pressure significantly, but when at Tianshu, Dachangshu at 1mA,2mA, 4mA,6mA and 8mA on β1-/-mice, decreased the pressure significantly(P<0.05 or P<0.01), suggesting an intensities-effects relationship between the intensities and the pressure at all four pressurepoints, and when the intensity reaches 4mA,the effect reaches a platform stage.4. EA stimulation at Quchi, Shangjuxu at 4mA,6mA and 8mA on β2-/-mice, increased the jejunal pressure significantly, but when at Tianshu at 6mA and 8mA, and Dachangshu at 4mA, 6mA and 8mA on β2-/-mice, decreased the pressure significantly(P<0.05 or P<0.01), suggesting an intensities-effects relationship between the intensities and the pressure at all four pressurepoints, and when at Shangjuxu, Quchi and Dachangshu at 6mA, the effect reaches a platform stage, but we didn’t find any platform stage when at Tianshu.Conclusion:1.There’s bidirectional regulation effect of EA stimulation in jejunum movement, and the underlying mechanism is mainly involved in the Autonomic Nerve Systerm(ANS). EA stimulation at Quchi, Shangjuxu can promote jejunum movement while at Tianshu, Dachangshu, jejunum movement is inhibited. β-adrenergic receptor, especialy (32 recepter, is involved in EA stimulation on jejunum movement.2. There exists intensities-effects relationship when EA stimulates jejunum movement, and the effect will reach a platform stage when stimulating quantity reaches a certain level. The mechanism is possibly involved in the different nerve segments and the activated peripheral afferent nerve between pressurepoints and the target organ, β-adrenergic receptor, especialy (32 recepter, is involved in the intensities-effects relationship between different peripheral afferent pathway activated by EA stimulation and jejunum movement.