| Objective:To study the possible pathogenesis of disorder of gastrointestinal motility diseases（DGIMD）based on the autonomic nervous system（ANS）,and use Hopu Sanwu Decoction as a breakthrough to study its role in regulating gastrointestinal motility mechanism and provide a theoretical basis for the clinical application of Houpu Sanwu Decoction.Methods:The first part:20 male SD rats were divided into a control group and a model group,10 rats in each group.The two groups of rats were injected with normal saline and L-arginine into the abdominal cavity respectively,and then the plasma motilin of the rats was measured.（MTL）,small intestine propulsion rate and gastric emptying rate,based on which to evaluate the success of the establishment of the model of gastrointestinal motility disorder in the model group.The second part:56 male SD rats that have passed the adaptation period were divided into 7 groups according to the computer numerical random method,with 8 rats in each group,namely the control group（KB）,the model group（MX）,the Magnolia officinalis Decoction group（P）,α-receptor inhibitor group（A）,α-receptor inhibitor+Houpu Sanwu Decoction group（AP）,β-receptor inhibitor group（B）,β-receptor inhibitor+Magnolia officinalis Sanwutang group（BP）.Except for the control group,the other 6 groups were modeled according to the aforementioned modeling method,and the control group was injected with an equal volume of normal saline.After the successful modeling and the completion of the corresponding drug intervention,the rat blood,stomach,jejunum,colon and other samples were taken,and the small intestine propulsion rate and gastric emptying rate were measured for some rats,and then the peripheral and local demethylation of the rats was studied.Epinephrine（NE）,α-type adrenergic receptors（α1,α2）,β-type adrenergic receptors（β1,β2）,norepinephrine transporter（NET）receptors and gene expression in tissues Effects of gastrointestinal motility disorders.Results:1.After intraperitoneal injection of L-arginine for 5 days,the gastric emptying rate,plasma motilin,small intestinal propulsion rate and body weight of rats in the model group were significantly lower than those in the control group.2.After the drug intervention,the small intestine propulsion rate and gastric emptying rate in the model group were lower than those in the Houpu Sanwu decoction group and theα receptor inhibitor group;the model group had the highest norepinephrine content in the stomach,jejunum and blood,The Houpu Sanwu Decoction group and the αreceptor inhibitor group were lower than the model group,but when the α receptor inhibitor was combined with Houpu Sanwu Decoction,the content of norepinephrine was abnormally increased.3.α 1 receptors have little relationship with gastrointestinal motility disorders;in the stomach,α 2 receptors have obvious changes,and the model group is significantly higher than other groups;β 1 receptors in the stomach and colon are significantly increased:β 2 receptor expression increased.4.Gastrointestinal motility disorder increases the expression of norepinephrine transporter in stomach and colon.Its mRNA expression also increased,andbegan to decrease rapidly witg the disappearance of modeling stressors.Conclusion:1.The intraperitoneal injection of L-arginine can build a 2-week aging rat model of gastrointestinal motility disorder.2.Adrenergic receptors（α 2,β1,β2）,norepinephrine transporter protein expression and gene expression increase,and the high content of norepinephrine in local tissues and peripheral blood leads to gastrointestinal motility disorders important reason.3.Houpu Sanwu Decoction may have an effect similar to α 2 receptor inhibitor in the stomach and colon,at the same time reduce the expression of adrenergic receptors,reduce the expression of norepinephrine transporter protein,and reduce the gastrointestinal tract The content of norepinephrine in local tissues and peripheral blood may be the reason for its effective treatment of gastrointestinal motility disorders. |
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