| Rheumatoid arthritis(RA) is a chronic and systemic autoimmune disease with major pathological features of arthrosynovitis. The clinical symptom is significantly centered of chronic, symmetrical, and polyarticular inflammation with recurrence in limb joints,such as hand and wrist. In addition it will be able to contribute to the destruction of bone, functional disorder of joint and even physical disability by accelerating the angiogenesis. Concurrently some other changes could happen with the its pathogenesis, including abnomalities in cardiac tissue, lung and kidney as well as gastrointestinal and neural aberrations.RA is the result of many pathogenic factors, which might have connections with inheritance, environment, psychological status, social causes and even smoking and alcohol. Intravaginal mycoplasma, as one of environmental factors, exerts effects on the genesis of RA. The mycoplasma, parasitizing vagina,can also lead to disease conditionally, when the intravaginal micro-environment is imbalanced due to low immunity and vaginal mucosa damage. However, few studies have reported it currently. This study was to investigate the component,serology characteristics and drug sensitivity of intravaginal mycoplasma, in order to reveal the relationships between female RA and intravaginal mycoplasma, provide guidance for clinical treatments and predict t he potential risks in advance.Methods1. Correlational study on female RA occurrence and intravaginal mycoplasma.Female groups were divided into RA patients(RA group), non-RA patients with other rheumatic diseases(non-RA group)and the healthy female(control group)in our study. Mycoplasma was cultured and subsequently we applied the Mycoplasma Typing Techniques to ensure mycoplasma infection and mycoplasma type, and then we analyzed the differences among three groups of different infection and components of mycoplasma, respectively to make the relationships clear.2.Drug sensitivity of female RA patients with intravaginal mycoplasma infection.The female RA patients were positive in mycomplasma infection,whose cervical secretions were taken out from-70℃ refrigerator and thawed, subsequently we conducted the drug sensitivity assay.3.Dynamic changes of serology in female RA patients with intravaginal mycoplasma infection and its clinical significance.The positive female RA patients in the first part assay,were again divided into group with mycoplasma infection(infection group) and that without mycoplasma infection(non-infection group). Afterwards, the relevant datum on serology and DAS28 and so on were collected and carried out the statistical analysis to understand the changes and evaluate its clinical values.Results1.The study contains 246 female RA patients, 113 non-RA patients with other rheumatic diseases and 126 healthy cases. There are 485 cases in total, 290 in positi vity, consequently the positive rate is 59.79%. Taking above datum into analysis,there was no statistical significance(P>0.05). Furthermore, the positive patients were classified in type. In the RA group of 144 cases, Ureaplasma urealyticum(Uu) accouts for 35.42%(51/144),3.47%(5/144)for mycoplasma homins(Mh) and 61.11%(88/144)for the Uu+Mh. In the control group of 80 cases,Ureaplasma urealyticum(Uu) makes up 57.50%( 46/80) 、 Mh 2.05%( 2/80) 、 Uu+Mh 40.00%( 32/80); Different infections between RA group and control group showed statistical significance(P<0.001).2.The postive in mycomplasma infection and typing assay are 290 cases totally, including 144 cases of mycomplasma infection, whose drug resistance rates are 10.42% of Josamycin 、 18.06% of doxycycline 、 22.22% of minocyline 、 28.47% of tetracycline 、 48.61% of clarithromycin 、 50.69% of azithromycin 、 61.11% of roxithromycin 、 61.81% of levofloxacin 、 64.58% of ofloxacin 、 71.53% of sparfloxacin、74.31% of erythrocin、88.19% of Ciprofloxacin.3.The number of cells in serum changes in infection group of mycoplasma with RA, including white blood cell(6.60±2.95),neutrophil granulocyte(4.38±2.61),lymphocyte(1.61±0.64),monocyte(0.46±0.23) and platelet(282.34×109±140.81×109), and that of non-infection group were6.01±2.08, 4.04±1.82, 1.43±0.63, 0.40±0.20, 237.26×109±98.39×109 respectively, shows significant difference(P < 0.05). The number of Eos and calcium concentration in infection group are 0.14±0.13)10^9/L and 2.17±0.12) mmol/L respectively, which is lower than that [0.17±0.22) 10^9/L 、(2.19±0.11)mmol/L] in non-infection group and the difference is significant.(P<0.05).Conclusion1.The infection rate of female RA patients with intravaginal mycoplasma was not significantly different from that of control group.2.The intravaginal mycoplasma infection types in female RA patients were significantly different from that in control group3. The intravaginal mycoplasma isless sensitive to Tetracyclines but highly or modestly to macrolide antibiotics4.Through anti-infection treatment with minocyline, the extent of disease activity of RA and ESR attenuated compared with that before treatment.5.After female RA patients infected with intravaginal mycoplasma, the number of blood cells including WBC、Neu、Lymph、Mon、PLT increased, while that of Eos and the concentration of Ca2+ decreased6.After female RA patients infected with intravaginal mycoplasma, there is no obvious changes in disease activity,ESR,CRP of RA... |
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