| Pathogenesis of ICP is complicated, many scholars believe that the state due to pregnancy, pregnant women in vivo estrogen, progesterone levels rise, affect the normal metabolism of bile acid, bile acid concentration increased and cause, serious can appear icteric, elevated liver enzymes, skin itching and other abnormalities. Motherland medicine thinks ICP belongs to "jaundice" disease category, according to dialectical ICP belongs to the "Yang huang". Synopsis of jaundice pulse license and cure "pointed out:" huang family income, from the wet ", the origin as well as from the outside feeling, also from the inside. Such as exogenous damp and hot plague poison, for wet from outside; Diet weary or YuZu wet delay after illness, belong to wet the endogenous. Because the origin indicates resistance medium, spleen and stomach health, diseases with stasis, pathogenesis is adverse, lose drain disorder caused by bile, bile not abide often, spillover skin bet and hair is yellow, yellow skin, bladder urine yellow card. Because of pregnancy the body blood aggregation does not leak, nourishes the fetus, thus causing the deficiency of yin and blood, Yang piansheng physique. If the patients have liver qi stagnation in the chest, not sparse, and because of lack of Qi of spleen deficiency, gathered together and wet; Liver Qi catharsis disorders, long Yu heat, damp heat stagnation of liver and gallbladder bile excretion and the formation of jaundice, too. So the treatment mechanism is soothing liver and invigorating spleen, clearing heat and removing dampness. "Jing Yue book," said yang jaundice:yellow card mostly due to spleen wet not shipped, stagnation and heat and become, must take the heat, heat dissipation Litong urination, urine clear, clear urine and jaundice. So many medical tended to Yinchenhao Decoction Treatment of ICP, and can receive a significant treatment effect. Many modern medicine according to the previous experience, to development, and on this basis, for the treatment of ICP patients and to observe the system, good clinical curative effect was also made. And modern medical research shows that, capillary artemisia can increase bile flow out, thereby promoting bilirubin excreted, significantly clear the hepatobiliary function of effective components in Scutellaria baicalensis; baicalin is, it also can promote the metabolism of bilirubin in speed, increase bile excretion to the in vitro rate. The theoretical foundation of the experimental basis for the Chinese medicine "the liver controlling dispersion" and "the kidney controlling reproduction", adopted by the ten traditional Chinese medicine eucommia,Eucommia bark, herba artemisiae capillaris, gardenia, rhubarb, radix scutellariae, mountain bund yellow, poria cocos, salvia miltiorrhiza, turmeric, cortex dictamni, these ten mixture of the Chinese medicine kidney qushi, oral ICP patients were given after the water decoction after sterilization. Yin Chen Keqing damp heat, hepatobiliary; gardenia efficacy of Qinggan Lidan and detoxification; Rhubarb can benefit the stool to vent stagnated heat, reduce bilirubin deposition in the liver and gallbladder. There was a significant decline in the role of TB, GC, ALT, AST. The jaundice, relieve itching to have obvious effect, can obviously reduce the occurrence of fetal distress and cesarean section rate, postpartum hemorrhage. The purpose of this experiment is the formation of cholestasis model rats and rats with estrogen induced liver cells, observe the effect of tonifying kidney and Eliminating Dampness Method expression party bile acid transport to the model protein Ntcp, clear kidney Qushi method prescription of Yinhuang mixture whether has the regulation of bile acid transport carrier related carrier protein expression of NTCP function in mRNA the level of expression is clear, Yinhuang mixture can regulate bile acid carrier related carrier protein ntcp. Molecular mechanism of kidney tonifying dampness Decoction "in the treatment of proven ICP may:may be achieved by expressing the main carrier protein transcription, associated with bile acid excretion and distribution of carrier regulation role. The experimental method for the use of 1 moI/L 17- alpha ethinyl estradiol to induce the formation of cholestasis model, the use of Tonifying kidney and eliminating dampness method medicine Yinhuang mixture to intervention treatment, using real-time quantitative PCR method and the WB to measure the level of Ntcp mRNA and protein expression. The dyeing method using HE to rat liver tissues fixed, made into pathological slices were observed under the microscope, in. The experimental result is the kidney Qushi method prescription of Yinhuang mixture can obviously increase the liver cells of Ntcp mRNA and protein expression level, P<0.05. Intervention can significantly relieve the liver injury of intrahepatic cholestasis in rats model of the use of Tonifying kidney and eliminating dampness prescription. Conclusion the molecular mechanism of Bushen dampness decoction treatment ICP probably by increasing the expression of bile salt transporter Ntcp messenger RNA and protein level of liver tissue to achieve. By increasing the expression and secretion of bile acid transport protein, is expected to become a new method in the treatment of ICP. |
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