| Global lifetime prevalence of schizophrenia is3.8‰-8.4‰, is the world’s7thlargest disease burden of disease, prevalence rate of Chinese population is4.13‰.Etiology and pathogenesis of schizophrenia has not yet clear, a lot of research onschizophrenia indicated that the genetic factors play an important role in itsoccurrence. Serotonin act on the postsynaptic target tissue or presynaptic tissuereceptors,large number of pathological and epidemiological studies have shown thatthe serotonin system have an association with schizophrenia, there are moreresearches on the relationship between serotonin1A receptor, serotonin2Areceptoror5-HTTLPR locate on transcription start site upstream of promote of serotonintransporter and schizophrenia, but the results are not inconsistent completely.Meta-analysis is a method of systematic and quantitative analysis depending onmultiple independent studies with the same research purposes,"reprocessing" theraw data.Increasing the sample size by combining multiple researches to overcomethe defects that sample size of individual study is small and the results of thesestudies are inconsistent, in order to obtain more objective and reliable results.ObjectiveTo investigate the association between HTR1A,HTR2A,5-HTTLPR genepolymorphism and schizophrenia, and to provide high quality evidence for the etiology ofschizophrenia.MethodsPubMed, EMbase, CNKI, WangFang and Vip information databases were used tosearch full text of all the relevant studies about the association betweenHTR1A,HTR2A,5-HTTLPR gene polymorphism and schizophrenia, which were published during January,2000to June,2014. Based on strict inclusion and exclusioncriteria, screening all retrieved studies, selecting, evaluating and accessing the data.RevMan5.1was used to perform the statistical analysis, regarded Odds Ratio(OR) asthe merged statistic, and selected the appropriate model based on the heterogeneitytest results. According to different ethnicities, the objects were divided into twosubgroups as European and Asian to analyze respectively. Also, depending ondifferent inheritances, the objects were divided into five patterns including allele,dominant, recessive, co-dominant and super dominant to analyze respectively, andanalyzed the sensitivity by eliminating the heaviest weight study. Stata12.0wasapplied to test Begg and Egger to assess publication bias.Results1. A Total of62studies about the association between HTR1A genepolymorphism and schizophrenia were searched and6studies were available for thisanalysis, including1278schizophrenia patients and1690controls. The Meta analysisresults showed that the distribution of HTR1A gene C1019G polymorphism betweenschizophrenia patients and controls had no significant difference. For total people, theallele OR=1.14(0.87-1.50), co-dominant model GG/CC, OR=1.26(0.81-1.95),dominant model GC+GG/CC, OR=1.11(0.78-1.58), recessive model GG/GC+CC,OR=1.19(0.97-1.47), super dominant model GG+CC/CG, OR=1.09(0.99-1.19). Thesensitivity analysis showed that the result was stable, Egger and Begg test resultsshowed that there is no publication bias among the researches.2. A Total of251studies about the association between HTR2A genepolymorphism and schizophrenia were searched and20studies were available for thisanalysis, including4109schizophrenia patients and3964controls. The Meta analysisresults showed that the distribution of HTR2A gene T102C polymorphism betweenschizophrenia patients and controls had no significant difference. For total people, theallele OR=1.05(0.95-1.16), co-dominant model CC/TT, OR=1.06(0.86-1.32),dominant model CC+CT/TT, OR=1.03(0.92-1.15), recessive model CC/CT+TT,OR=1.03(0.92-1.15), super dominant model CC+TT/CT, OR=0.96(0.88-1.05). No differences in the distribution of genes in different ethnic populations subgroups. ForEuropean, the allele OR=1.12(0.91-1.37), co-dominant model CC/TT,OR=0.98(0.81-1.19), dominant model CC+CT/TT, OR=1.28(0.94-1.75), recessivemodel CC/CT+TT, OR=1.07(0.92-1.24), super dominant model CC+TT/CT,OR=0.94(0.82-1.07). For Asian, the allele OR=0.99(0.91-1.08), co-dominant modelCC/TT, OR=1.19(0.78-1.81), dominant model CC+CT/TT, OR=1.09(0.87-1.37),recessive model CC/CT+TT, OR=0.99(0.84-1.17), super dominant model CC+TT/CT,OR=0.98(0.87-1.11).The sensitivity analysis showed that the result was stable, Eggerand Begg test results showed that there is no publication bias among the researches.3. A Total of65studies about the association between5-HTTLPR genepolymorphism and schizophrenia were searched and9studies were available for thisanalysis, including1738schizophrenia patients and2012controls. The Meta analysisresults showed that the distribution of5-HTTLPR polymorphism betweenschizophrenia patients and controls had no significant difference. For total people, theallele OR=1.04(0.73,1.48), co-dominant model SS/LL, OR=1.02(0.60,1.73), dominantmodel SL+SS/LL, OR=0.95(0.63,1.42), recessive model SS/SL+LL, OR=1.05(0.70,1.58), super dominant model SS+LL/SL, OR=1.20(0.82,1.74). No differences inthe distribution of genes in different ethnic populations subgroups. For European, theallele OR=0.78(0.49,1.25), co-dominant model SS/LL, OR=0.71(0.35,1.43), dominantmodel SL+SS/LL, OR=1.28(0.83,1.99), recessive model SS/SL+LL, OR=0.78(0.50,1.20), super dominant model SS+LL/SL, OR=1.13(0.62,2.06). For Asian, theallele OR=1.31(0.79,2.15), co-dominant model SS/LL, OR=1.49(0.70,3.18), dominantmodel SL+SS/LL, OR=0.74(0.40,1.36), recessive model SS/SL+LL, OR=1.35(0.73,2.48), super dominant model SS+LL/SL, OR=1.25(0.72,2.17).The sensitivityanalysis showed that the result was stable, Egger and Begg test results showed thatthere is no publication bias among the researches.Conclusions.There was no significant association between5-HT1A receptor gene C1019Glocus polymorphism and schizophrenia. 2.There was no significant association between5-HT2A receptor gene T102Clocus polymorphism and schizophrenia.3.There was no significant association between5-HTTLPR gene polymorphismand schizophrenia. |
