The Role Of Fli-1in Progression Of Hepatocellular Carcinoma And Its Effect On Prognosis

Objective The aim of this study was to investigate the expression of Fli-1in hepatocellular carcinoma (HCC) tissues and the adjacent tissues,and toanalyse the relation between Fli-1expression and clinicopathologicalparameters, and the effect of Fli-1expression on patient prognosis.Methods The tumor tissue and clinical information of the patients withhepatocellular carcinoma were collected from January2011to December2012in the first hospital of Jilin University. The clinical data were retrospectivelyanalyzed. Fli-1antibody labeling of tissue samples by immunohistochemicalmethod. The results used statistical analysis by non parametric test,Kaplan-Meier survival curve and Cox regression.Results A total of73patients with hepatocellular carcinoma were enrolled inthe study.The expression of Fli-1in HCC tissues was significantly higher thanthat in adjacent tissues, P=0.003. The expression of Fli-1in the tumor cell isassociated with HBV infection (P=0.023) and pathologic differentiation(P=0.014), high expression of Fli-1in the cancerous tissues with HBV infectionand low pathological differentiation. Fli-1negative or positive in adjacentsegment is associated with the intensity of Fli-1expression in HCC tumor cell.The Kaplan-Meier survival curve showed that the low expression of Fli-1in tumor cell and negative expression of Fli-1in adjacent segment had better PFS(P=0.039and P=0.023) in BCLC0/A patients, but OS (P=0.138and P=0.251)had no statistical significance difference (P); multiple Cox regression analysisshowed that the expression of Fli-1in tumor cell and adjacent segment had trendto effect on the PFS（HR=2.013；95%CI：1.088~3.724；P=0.026and HR=4.471；95%CI：1.179~16.962；P=0.028）, but this result had no statistical significancedifference in OS（HR=1.192；95%CI：0.522~2.724；P=0.677and HR=1.937；95%CI：0.522~7.185；P=0.323).Conclusion1. The expression of Fli-1in normal liver cell, HCV positive livercell and liver metastasis of gastrointestinal tract tumor is negative, and in HBVpositive liver cell is scattered weak positive. Both in HCC cancer tissues andadjacent tissues show positive expression of Fli-1, located in the nucleus, andthe expression in cancer tissues was significantly higher than that in adjacenttissues;2. The expression of Fli-1in HCC is correlated with HBV infection andpathological differentiation in cancer tissue. Fli-1negative or positive inadjacent segment is associated with the intensity of Fli-1expression in HCCtumor cell;3. For patients in BCLC0/A stage, high expression of Fli-1in cancertissue is a bad factor of PFS. The expression in the adjacent tissues suggests ahigh risk of recurrence. At the same time, the expression of Fli-1in cancertissues and adjacent tissues can be an independent factor of PFS.