Studies On The Efficacy Of Shengefentong Capsules On Diabetic Neuropathy And Its Mechanism

Objective:The study is aimed to investigate the effects and its mechanisms of shengefentong capsule (SGFT) on diabetic neuropathy (DPN).Methods:Mice diabetic neuropathy were induced by ip streptozotoein (STZ)150mg/kg. The normal control groups were intraperitoneal with the same dosage of citrate bu ffer. The mice blood gl-ucose was higher than16.65mmol/L (72h later) were rando mly divided into model group, Morphine Hydrochloride injection0.01g/kg, Mudan pa rticle3.5g/kg, SGFT0.3g/kg, SGFT0.6g/kg,SGFT1.2g/kg. Po, once a day for2weeks. Models, Morphine hydrochloride injection group and normal controls were give n equal volume of solvent. Mice of Morphine hydrochloride injection group were intra perited with the dosage drug at the last delivery time.1h after the last treatment, the pain threshold(s) of the mice were measured by thermal-stimulation or pressure-stimul ationt.The type2diabetic rats were induced by high-fat-diet and iv STZ25mg/kg, T he normal controlgroup (NC) were intraperited with the same dosage citrate buffer.The se rats blood glucose were higher than13.7mmol/L were randolnly divided into mod elgroup, Metforinin Hydrochl-oride0.25g/kg), Xiaokewan1.25g/kg, SGFT0.1125g/k g, SGFT0.225g/kg, SGFT0.45g/kg. Po, once a day for8weeks. Models and norm al controls were given the equal volume of solvent. After the last administration, rats sciatic nerve local blood flow quantity and blood rheology indexes were measured; sci atic nerves were observed using microscope and electron microscopy. The levels of ser urn glucose (GLU), low density cholesterol (LDL), high density cholesterol (HDL), tot al cholesterol (TC) and triglyceride (TG), superoxide dismutase (SOD), malondialdehyd e (MDA) and c-peptide (C-P), insulin (INS), advanced glycation end products (AGEs) and blood glycosylated hemoglobin were measured in rats. The pancreas pathological morphology were detected under microscope.In addition, the effect of SGFT on the levels of blood glueose in normal mice and type1diabete mice were further studied.Results:The results showed that the sensitivities of thermal-stimulation and mechanical-stimulation in type1diabetes mice were higher than the normal animals. There was a significant difference between model and normal control group.SGFT can significantly reduce the sensitivities of thermal-stimulation and mechanical-stimulation in diabetes mice.The flux of the sciatic nerve local blood flow was slowed down in type2diabetes rats. SGFT0.225g/kg group can significantly improve the local blood flow changes in sciatic nerve in type2diabetes rats.The results the blood rheology indexes in diabetic model group rats showed that blood viscosity was increased. SGFT0.1125g/kg and0.225g/kg can increase the whole blood low shear viscosity (10), whole blood viscosity (60), whole blood high shear viscosity (150) and erythrocyte aggregation index, and SGFT0.45g/kg significantly reduced the blood viscosity, but has no effect on the erythrocyte aggregation index.The levels of HDL, LDL, TG, TC increased, but the insulin level dropped significantly, while the levels of HbAlc,MDA, SOD and C-P there were no changes in serum in type2diabetes rats,. SGFT could significantly reduce the levels of LDL and TG in type2diabetes rats.SGFT had no effect on the levels of blood glucose in normal mice, type1diabetes mice or type2diabetes rats.Conclusion:SGFT can reduce the sensitivity of pain, improve morphology changes of the sciatic nerve in the diabetes animal, confirming that SGFT has a protective effect on the structure and function of diabetic neuropathy nerve. SGFT can improve blood lipid metabolism disorders and sciatic nervous’local blood flow and blood rheology indexes, suggesting that mechanism of the SGFT may be related to adjust blood lipid metabolic disturbance, improve the symptoms of vascular ischemia, hypoxia and injury of microcirculation.