Internal Medicine Of Traditional Chinese Medicine

Objective: To observe the protective effect of compound shenglong capsule onthe adriamycin induced kidney damage in rats and its mechanism of loweringproteinuria by biochemistry, histochemistry and pathological methods.Methods:100healthy male SD rats, weight(180-220)g, adaptive feeding for oneweek,10rats were selected as control group randomly,90rats were twice injected asmall dose of intravenous injection doxorubicin to rebuild the model of renalinjury,after modeling successfully the rats (except the control group) were randomlydivided into5groups,model group,positive control group, the experimentalgroup(compound shelong capsule low,medium and high dose groups), withlenggongteng tablets as a positive control,continuous oral administration for sixweeks, and observe the shelong capsule on adriamycin induced by the generalconditions and urinary protein excretion in rats after kidney injury.After the lastadministration left the rat urinary microalbuminuria(mALB),urinary α1-microglobulin(α1-MG), urinary enzyme N-acetyl-β-glucosaminidase(NAG); ratsafter anesthesia,abdominal aortic blood,erum preparation,chemical colorimetricdetection of serum albumin(ALB),triglycerides(TG), cholesterol(CHO), aspartateaminatranferse aminotransferase(AST), alanine aminotransferase (ALT), bloodcreatinine(Scr), blood urea nitrogen (BUN) and other biochemical indicators; isolated renal tissue, the left part of the preparation of kidney tissue homogenates, ELISA wasused to detect kidney tissue interleukin-6(IL-6), transforming growth factor-β1(TGF-β1); right renal tissue stored at-80℃for immunohistochemical observationof vascular endothelial growth factor(VEGF), kidney disease protein(Nephrin)expression of heparan sulfate proteoglycan (HSPG); light microscopy and electronmicroscopy observe the kidney tissue changes in general morphology andultrastructure. Application SPSS21.0statistical software for statistical analysis.Results: After administration for two weeks, compared with the control group,model group24-hour urinary protein excretion was significantly increased,thedifference was statistically significant (P<0.05), to compared with model group, thetreatment groups had lower proteinuria, the difference was not statistically significant(P>0.05),4,6weeks after administration, compared with the model, each treatmentgroup was significantly reduced proteinuria, the difference was statisticallysignificant(P<0.05or P<0.01); compared with the control group, rat urine modelgroup α-MG, NAG and mALB significantly increased,the difference was statisticallysignificant(P<0.01), compared with the model group, positive control group andtrial, the high dose group urine α-MG, NAG significantly reduced,the treatment groupwere significantly lower urine mALB, the difference was statisticallysignificant(P<0.05or P<0.01). To compared with the control group,serum TG ratmodel group,CHO increased significantly,ALB was significantly lower, thedifference was statistically significant (P<0.01); to compared with the modelgroup,positive control group and compound snake dragon made of high-dosegroup serum TG, CHO significantly reduced,the treatment group was significantlyhigher serum ALB, the difference was statistically significant (P<0.05or P<0.01). Tocompared with the control group,model group, renal tissue IL-6,TGF-β1was significantly increased,the difference was statistically significant (P<0.01), tocompared with the model group, positive control group and the test, the high dosegroup kidney tissue IL-6,TGF-β1was significantly increased, the difference wasstatistically significant (P<0.01); compared with the control group, the renal tissue ofrats in the model group was significantly higher VEGF, HSPG, Nephrin significantlylower,the difference was statistically significance (P<0.01), to compared with themodel group,positive control group and the test medium and high dose groups ofVEGF in renal tissue was significantly reduced,significantly increased HSPG andNephrin, the difference was statistically significant (P<0.01). Each group ofhistological changes in rat kidney morphology:light microscope model groupincreased mesangial cells, mesangial matrix,tubular dilatation, basement membranethickening power microscopic model group rats,increased mesangial cell lines, withmatrix hyperplasia, extensive fusion of foot processes.Experimental high dose grouphad significant protective effect on renal injury morphology.Conclusion: The mechanism of compound shelong capsule reduce protein maybe reducing IL-6, TGF-β1, inhibition of VEGF, enhanced HSPG, Nephrin expressionof immune and inflammatory damage mitigation,the glomerular filtration barrier.