| Paraquat poisoning was a serious disease, which will cause multipleorgans damaged. Its onset is quick and fatality is very high. At present, thestudy of lung injury by paraquat poisoning has been quite mature, but itdoes not significantly decrease the mortality. The study of kidney damageand other organ damage are relatively few, actively exploration of paraquatpoisoning of kidney and other organ damage can be helpful to the diagnosisand treatment of paraquat poisoning. This experiment will use the320-rowCT perfusion scan to observe rabbit’s renal hemodynamic changes withthe paraquat poisoning in early stage and evaluate renal injury by perfusionparameters data analysis. The poisoned abbit was injected ulinastatin tostudy the protective effects of ulinastatin on kidney damage induced byparaquat poising. To further exploration of the mechanism of renal injurycaused by paraquat poisoning, can provide a theoretical basis for clinicaltreatment.Forty-eight healthy New Zealand white rabbits were divided intoparaquat poisoning group, ulinastatin group and normal saline controlgroup in this study, with sixteen rabbits in each group. Paraquat poisoninggroup and ulinastatin group were given a single dose of paraquat solution35mg/kg by intraperitoneal injection, control group was given the samevolume of physiological saline injected in peritoneal cavity. The ulinastatingroup received the intravenous injection of ulinastatin100Ku/kg afterinjection of paraquat for1hour, then the same dose of ulinastatin was givenevery day, for three days.The control group and the paraquat poisoning group were given the same volume of physiological saline of0.9%. Eachgroup was given320row CT perfusion scan after injection of2,12,24,48,72h respectively, obtain perfusion images, Renal Blood Flow (Renal BloodFlow, RBF) and Renal Blood Volume (Renal Blood Volume, RBV)perfusion parameters every time point.2ml blood was collected from earmarginal vein for detecting serum creatinine (Cr) and urea nitrogen (BUN)every time point. Observe the histopathological changes of the renal tissuewere stained with HE from two rabbits in each group. The study shows thatthe detection of control group has no significant changes. Paraquat group:Serum Cr and BUN after injection paraquat2h were increased significantlyat24,48,72h (P<0.05). RBF and RBV after injection paraquat2h showedno significantly change compairing with control group too, but were lowersignificantly than control group at12,24,48,72h (P<0.05). The differencewas statistically significant. Ulinastatin group: compared with the infectedgroup,2,12h, two time points, serum creatinine and urea nitrogen, renalblood volume and renal blood flow have no obvious improvement; Serumcreatinine, renal blood flow were improved obviously at24,48,72h, thedifference was statistically significant (P<0.05); Urea nitrogen and renalblood volume at48,72h two time points were improved significantly, thedifference was statistically significant (P<0.05). Observed by lightmicroscope, Renal organizational structure was clear in Saline group, Thereare no cell congestion and edema, degeneration pathology. Paraquat groupshowed pathological changes in glomerular capillary endothelial cellcongestion and edema, vacuolization, proximal tubular epithelial cellswelling, vacuolar necrosis and so on, with the time extension, the damageincreased. Ulinastatin group: With the time prolonged, the renal injuryexacerbation increased comparing with the paraquat group, the renal injurywas relieved at48,72h.This study shows that320-row CT can evaluate renal damage degreeof rabbit with paraquat poisoning by perfusion pseudo color image in the early stage.At the same time, according to the perfusion parameters it canjudge the damage degree. Ulinastatin had a protective effect on rabbitkidney damage induced by paraquat poising, by improving circulation andpromoting paraquat elimination;320-row CT perfusion scanningtechnology can reflect the degree of kidney damage earlier than the clinicaldetection of creatinine and urea nitrogen. |
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