Effect Of HGF And Truncated TGF Beta Receptor Ⅱ On Wound Healing

BackgroundScar formation in wound healing process is one of the clinical problem. Scar treatment include surgical resection, radiotherapy and drug treatment. But the effects are not ideal, not only easy to relapse, but also more likely to develop into hypertrophic scar. A large number of previous studies have demonstrated TGF-P plays a key role in the process of scar formation, which can not only accelerate the proliferation of fibroblasts and the division, but also promotes gene expression and protein synthesis of collagen. Truncated TGF-β type Ⅱ receptor (t-TGF-β R Ⅱ) is a TGF-β RⅡ which lost its fragments rich in serine and glycine. t-TGF-βRⅡ is not able to continue signal transduction, but still capable of binding to TGF-β, thereby blocking TGF-β signaling pathway.Hepatic growth factor (HGF) is a multifunctional growth factor, it can promote mitosis, anti-apoptotic, anti-fibrosis, promoting angiogenesis, inhibition of fibrosis in hepatic tissue, and the generation of TGF-β to enhance collagenase activity.Theoretically, t-TGF-βⅡ and t-TGF-P would be effective synergy promoting wound healing and reduces the formation of collagen.Aim1) Research the expression of lentivirus loaded t-TGF-β RⅡ and HGF. 2) Study the effect on expression of collagen Ⅰ,Ⅲ of t-TGF-β RⅡ and HGF.3) Effects of the expression of t-TGF-β RⅡ and HGF in wound healing and scar formation.MethodsUsing lentivirus-mediated double gene (t-TGF-β RⅡ/HGF) complexes transfected into mouse embryonic fibroblasts NIH-3t3cells, set the empty virus group (lentivirus), t-TGF-β RⅡ control group (t-TGF-β RⅡ-lentivirus), HGF group (HGF-lentivirus), the experimental group (t-TGF-P RⅡ-lentivirus, HGF-lentivirus), test cells of each sample t-TGF-β RⅡ and HGF in the expression levels of mRNA and protein level.In the back of the rat model for wounds caused by scarring in the48hours prior to surgery in the experimental group pre-injection fluid beneath the incision virus, the control group was injected with t-TGF-β RⅡ virus solution, HGF virus solution, bare virus solution, DMEM culture medium. Respectively, in one week, two weeks, when the rats were sacrificed, specimens are measured with general observation, pathology, and collagen detection.ResultsExpression of t-TGF-β RⅡ and HGF in NIH-3t3cells of experimental group was significantly higher than the control group (p<0.01).In experimental group, expression of collagen Ⅰ,Ⅲ was significantly reduced compared with the control groupsConclusion 1) Target gene can be efficiently transduced by lentivirus, and can be stably expressed in a cell for a long time.2) t-TGF-β RⅡ and HGF mediated by the virus can be over-expressed in NIH-3t3cells.3) t-TGF-β RⅡ inhibit the synthesis of collagen Ⅲ in NIH-3t3cell Ⅰ, but not obvious with HGF.