Preparation Of Bacitracin-PBCA Nanoparticles Eyedrops

Objective Bacitracin is a broad-spectrum antibiotics, its antibacterial spectrum similar to penicillin, can strongly inhibit the Gram-positive bacteria, of penilillin-resistant Staphylococcus aureus also had a stronger inhibitory effect. Its mainly used in clinical penilillin-resistant Staphylococcus aureus infection and external to the skin infections. But, bacitracin has a short half-life, unstable nature of disvantage. Therefore, this experiment will be proposed to use modern preparation means to be made into nanoparticles preparations, to reach the purposes of improving drug stability, prolonging drug half-life, enhancing the curative effect. Provides the reference for the preparation for the further study bacitracin.Methods Preparating the Bacitracin-PBCA nanoparticles by emulsion polym erization method; Selecting the inclusion rate of Bacitracin as the control index to optimize preparation method, exploring the L9(34) orthogonal design test, selecting the drug weight to BCA volume, poloxamerl88weight plus dextran70weight, pH value as the investigation facts. And evaluating quality of nanoparticles. HPLC method was used for the determination of drug content in nanoparticles. And make the pharmacodynamics study for nanoparticles eye drops.Results The optimal preparation technology of Bacitracin is as follows:drug weight to BCA volume was1:10(mg:μl), poloxamerl88weight plus dextran70weight was30mg, pH value was2. Nanoparticles obtained is the rules of spherical, uniform size, no adhesion.The average particle size was149.7nm, The average encapsulation efficiency was85.75%. pharmacodynamic experiment confirmed bacitracin nanoparticles eye drops for bacterial keratitis agent has good curative effect.Conclusion The preparation technology of bacitracin-PBCA nanoparticles is easy,the quality meets the requirements, the stability is good. Bacitracin-PBCA nanoparticles eye drops meets quality standards.The pharmacodynamic experiment confirmed bacitracin-PBCA nanoparticles eye drops has a good treatment role for bacterial keratitis, at the same time can slowly release, reduce drug administration times.