Studies On Bacitracin Liposome Eye Drops

Objective:Bacitracin is an eye infection peptide drugs with good treatment effect. its inhibitory effect is significant. Similar to penicillin, can strongly inhibit the Gram-positive bacteria also have a strong inhibition of penicillin-resistant Staphylococcus aureus. The major clinical presentations for penicillin-resistant staph infection and foreign for skin infections. Bacitracin hydrophilic drugs, but its solution is unstable, readily biodegradable, low bioavailability, short half-life. Therefore made of bacitracin liposomes, the drug between wrapped in film to avoid contact with the aqueous solution, increasing its stability, to extend the time of contact with the cornea, improve the outcome. For clinical application to provide a safe, convenient and effective new drug formulations.Methods:In this study, bacitracin liposomes prepared by reverse evaporation method, using the Lq (3+) orthogonal design to optimize the liposome technology, evaluation indicators, soy lecithin and phospholipids. drugs and fat to liposomes was The quality of the mass ratio of phosphate buffer pH3factors investigated factors. And liposomes evaluate the quality of. Using high performance liquid chromatography determination of drug content in liposomes and liposome eye drops pharmacodynamic inspection.Results:The optimal process of the preparation of bacitracin liposomes of soybean phospholipids and cholesterol mass ratio of4:1. the quality of drugs and lipid ratio1:30, phosphate buffer pH7.4. Liposomes obtained for the particle size of the circular structure of the uniform, no adhesion to the average particle size of220nm, the potential value of-37.03mV.. The average encapsulation efficiency was87%. Long-term irritation test and continuous irritation test showed that the liposome eye drops of bacitracin non-irritating to the eye, and pharmacodynamic trial confirmed that the better treatment of bacitracin liposome eye drops caused by Staphylococcus aureus of keratitis.Conclusion:Bacitracin liposome technology, the quality of research to meet the requirements, good stability, bioavailability. and low cost. Pharmacological experiments confirmed that bacitracin liposome eye drops on corneal bacterial infection, has a good therapeutic effect, and also has the characteristics to reduce delivery times.