Effects Of Electronic Acupuncture At Abdominal Points On The Blood Lipids And Serum Adiponectin In Rats With Simple Obesity

Objective：To observe the influence and explore the effect of the BMI、the bloodlipids、the ADPN and the the morphology of fat cells of the rats through the acupuncture atabdominal points such as Tianshu、Zhongwan、Guanyuan、Daheng on rats with simpleobesity via the establishment of rat model of simple obesity,So we can Clear themechanism of lipid,and Provide a clear basis laboratory for losing in clinical.Methods：10male SD rats of clean grade were randomly selected from the100SDrats(the weight is16010g)as the normal group,which were fed with normal stuff,whileother90rats were fed with high-fat diet.Eight weeks later,30rats which are selected fromsuccessful modeling whose Standard of is that molding group rats’ body weight is twentypercent higher than that of normal group were randomly divided into3groups with10ratsin each group：The mode group、electro-acupuncture group1(Tianshu、Zhongwan、Guanyuan、 Daheng),electro-acupuncture group2(Housanli、 Sanyinjiao、 Zhongwan、Guanyuan). Making the acupuncture treatment on electro-acupuncture groups for20daysafter the model is successful, during which the normal groups and model Group ratscrawling at the same time.At the end of the model respectively,every weak during thetreatment before put to death. special experimental body should measure and compare theweight and body length(the length from the nose to the anus)of the rats.All the rats must befasted for24hours after the treatment and be executed on the22nd day of the treatment.We can get the blood from the abdominal aorta and put it in to Centrifuge,so that we canget blood serum,from which we can observe the changes of body fat.Enzyme-linkedimmunosorbent assay should be used to detect the change of the content of serumadiponectin. A fixed little piece of adipose tissue should be got from the door of left kidneyand fixed it in the formalin which Concentrations is10%. pathologic slices should be doneParaffin-embedded and then be staining.after all we can observe the changes in the size ofthe diameter of the abdominal wall fat cells under the microscope.Results：(1)The weight of body and the weight of the fat around kidneys and aroundepididymides of the fats of the treated group are significantly different between that ofmodel Group,and that of the treated group is significantly lower that of model Group(P<0.01or P<0.05);Comparison between electro-acupuncture group1and two,we have not seen a significant difference(P>0.05),during the weight of body and the weight of thefat around kidneys and around epididymides of the rats.But the1set have a better trendthan the2set in weight loss.(2)The TG content of the electro-acupuncture group issignificantly lower than that of the model Group(P<0.05),but the TG content of theelectro-acupuncture group1is not significantly different between the electro-acupuncturegroup2(P>0.05);The content of CHO、LDL of the treatment group is significantly lowerthat of the model Group(P<0.01or P<0.05),but that of the electro-acupuncture group1hasno significant differences between that of the electro-acupuncture group2(P>0.05);Thecontent of HDL of the treatment group is significantly higher than that of the modelGroup(P<0.01or P<0.05),but that of the electro-acupuncture group1has no significantdifferences between that of the electro-acupuncture group2(P>0.05);(3)The content ofadiponectin in serum of the model group obviously reduced than that of the normal group(P<0.01),but that of the treatment group is significantly higher than that of the modelgroup(P<0.01orP<0.05);though,the content of adiponectin in serum of the electro-acupuncture group1has noobvious differences between the group2,but the1set has a better trendin regulation of the function of the lipid metabolism-related hormone levels.(4)Thediameter of the fat cells around kidneys of the model group is much bigger than that of thenormal group under the light microscope,which indicates the model is successful;Thediameter of the fat cells around kidneys of the electro-acupuncture group is smaller thanthat of the model group after treatment,which point out that the acupuncture at abdominalpoints such as Tianshu on rats with simple obesity may reduce the diameter of the fatcells,but that of the electro-acupuncture group1is closed to group2.Conclusion：The weight of body and the weight of the fat around kidneys andaround epididymides and the blood lipid of the fats of the electro-acupuncture group aremuch decreased than that of the model group,but the content of adiponectin in serum of theelectro-acupuncture group is obviously higher than that of the model group via thediameter of the fat cells around kidneys significantly decreased,all of which show the theeffect of the treatment,after the treatment of the acupuncture at abdominal points on of ratswith simple obesity.The difference between the electro-acupuncture group1(Tianshu、Zhongwan、Guanyuan、Daheng)and group2(Tianshu、Zhongwan、Guanyuan、Daheng)hasno significant meaning,but the1set have a better trend than the2set in weight loss.