The Research Of HBV Genotype Distribution And Mutation Between Hui And Kirgiz Population In Xinjiang

Objective: To study quantitation and distribution analysis of hepatitis B virus (HBV)genotypes of Hui and Kirgiz people in Xinjiang, and explore the relationship between HBVgenotype and mutation of the C region.Methods:1.Serum samples were collected from HBsAg carriers of Hui and Kirgizpeople in Xinjiang. Real-time genotyping and quantitative PCR (GQ-PCR) and the nextgeneration sequencing (NGS) were used to detect HBV genotyping, while real-time PCRmethod for quantitative detection of the HBV DNA load.2. To explore the characteristics ofHBV genotypes in Hui and Kirgiz people, the nucleotide homology alignment between thesequencing results of HBV C region and the HBV GeneBank reference strains was conductedby bioinformatics, use phylogenetic tree to determine variation law in HBV C region.3.Toanalyze the relevance of each genotype and chronic hepatitis c zone variation and Chronichepatitis b disease.Results:1.A total of211patients with chronic hepatitis B which included100Hui and111Kirgiz people were collected. All serum samples were validated as HBsAg positive byELISA.2. HBV genotyping results:94samples in Hui people were successfully genotyped.Among them,7patients (7.44%) had genotype B,62patients (65.95%) of had genotype C,20patients (21.27%) had genotype D,5patients (5.32%) had genotype mix. While99Kirgizsamples were successfully genotyped, including10patients (10.10%) of type B,6patients(6.06%) of type C,75patients (75.76%) of type D, and8patients (8.08%) of genotype mix.The results show that type C was the dominant type of Hui people, while to Kirgiz peopletype D was their advantage.3. HBV-DNA quantitative test results showed:185patients hadHBV-DNA with quantity ranging from3~10log10copies/ml, and mean and standarddeviation of6.51±1.13log10copies/ml in Hui and5.671.04log10copies/ml in Kirgizpeople, and the distribution of DNA load has significantly difference among high,mediumand low copy groups.4.The results of gene mutation analysis showed that the mutant formsand frequencies of BCP and BP regions were different among B, C, and D genotypes, the HuiA1762T and G1764A mutation rate is the highest in C genotype (45%and46%), followedby D-type (28%) and B-type (14%); while G1896A, G1899A mutation rate to the highestgenotype D (66%and19%). D genotype mutation frequency in Kirgiz was similar with Huipeople.Conclusion: The distribution of hepatitis B virus genotype of Hui and Kirgiz people inXinJiang has the nation’s own characteristics, and there are significant differences betweenthe correlation of their genotype and C region mutation. Results suggest that different control strategies should be taken to prevent and control hepatitis B in the multi-ethnic region inXinJiang, which should be based on the characteristics and laws of HBV genotype and itsmutation of different ethnic groups, so as to formulate targeted prevention and controlmeasures, reduce the prevalence and incidence of hepatitis B of all ethnic groups in XinJiang,improving the quality of health level of all ethnic groups in XinJiang.