| Although Rotavirus (RV) infection is a kind of self-cure disease, the infants is thedominating infected group, the immune system of the infants has not yet completelymature, and with higher infection rate, it is a generalized disorder affecting otherorgans in severe cases. Present foreign and domestic research showed that RV antigenor RV DNA can be detected in peripheral blood of children with diarrhea caused byRV infection, which confirmed that the rotavirus can lead to extra intestinal infectionin certain circumstance. Animal experiments have shown that hepatic pathologicalchanges emerged in suckling mice of severe rotavirus infection group after execution,humeral immunity and cellular immunity exception as well as variety changes ofcytokine expression can also be found at the same time. Several cytokines excretedby Th1and Th2cell play important roles in pathogenesis and development process ofrotavirus infection. Several cytokines with few reports and were closely related toliver injury induced by RV infection: interleukin-6(IL-6), interleukin-10(IL-10),tumor necrosis factor-（TNF-） and interferon-γ （IFN-γ）were selected in thisexperiment to discuss the pathogenesis of diarrhea and liver damage caused byrotavirus.Objective:this study provide experimental basis for treatment and prevention of rotavirusinfection in infants by comparing the protein expression of IL-6, IL-10, TNF-andIFN-γ in the serum of children with diarrhea and liver injury caused by rotavirus, inthe serum of children with diarrhea and was not associated with liver injury caused byrotavirus, as well as the serum of normal children, with the combination of mRNAexpression of cytokines in serum and liver of suckling mice and analysis of the role of cytokines in the pathogenesis of diarrhea and liver injury caused by rotavirus.Methods:The serum ALT values of112cases of infants with diarrhea caused by rotaviruswere detected in hospital last year, all infected children were divided into three groups:53cases of children with diarrhea and liver injury caused by rotavirus,59cases ofchildren with diarrhea and was not associated with liver injury caused by rotavirus,25cases of normal children with a medical examination in the same period. All childrenwere underwent venous blood collection and the protein expression levels of IL-6,IL-10, TNF-and IFN-γ were detected by ELISA kit. The extra intestinal infection ofrotavirus in neonatal rats model was established, eyeball blood and liver tissues werecollected after execution. The morphological changes of the liver were observed byHE staining method and the protein expression levels of IL-6, IL-10, TNF-andIFN-γ in serum and liver were detected respectively by ELISA method andimmunohistochemical method. The mRNA expression levels of IL-6, IL-10, TNF-and IFN-γ in liver tissue were detected by RT-PCR. The expression levels of variouscytokines were compared by statistical analysis and the relationship of diarrheacaused by rotavirus and liver injury will be estimated.Results:1. The protein expression results of IL-6, IL-10, TNF-and IFN-γ in the serumof infants:(1) The protein expressions of IL-6, IL-10, TNF-and IFN-γ in serum ofchildren infected with rotavirus were all significantly increased, the difference wasstatistically significant (P<0.05or P<0.01). The levels of IL-6, IL-10, TNF-andIFN-γ in the restoration stage decreased slightly, IL-6, IL-10and IFN-γ were reducedsignificantly, but they all did not reach the normal level.(2) The expression levels of IL-6and TNF-in serum of children with mediumor severe diarrhea infected with rotavirus increased significantly (P<0.05or P<0.01)compared with those with mild diarrhea symptoms, while the difference of IL-10and IFN-γ in serums of children with different degree of diarrhea was not significant(P>0.05).(3) The protein expression levels of the four cytokines in serum of childreninfected with rotavirus were remarkably higher than those in normal children, thedifference was statistically significant (P<0.05). The protein expression results of IL-6,IL-10and TNF-in children with diarrhea and liver injury caused by rotavirus wereremarkably higher than those in children with diarrhea and was not associated withliver injury caused by rotavirus, and the difference was statistically significant(P<0.05). There was no significant difference between the expression level of IFN-γin children with diarrhea and liver injury caused by rotavirus showed no significantdifference and in children with diarrhea and was not associated with liver injurycaused by rotavirus.(4) The expression levels of IL-10in serum of children with medium or severediarrhea and the levels of TNF-in serum of children with severe diarrhea weresignificantly increased compared with those with mild diarrhea symptoms, thedifference was statistically significant (P<0.01).2. The protein expression results of IL-6, IL-10, TNF-, IFN-γ and mRNAexpression level in serum and liver of suckling mice:（1）The protein and mRNA expression levels of IL-6, IL-10, TNF-, IFN-γ inserum and liver tissue of suckling mice were higher than those in normal groups.（2）The protein and mRNA expression levels of IL-6, IL-10and TNF-inserum and liver tissue of suckling mice with diarrhea and liver injury caused byrotavirus were remarkably higher than those in the suckling mice with diarrhea andwas not associated with liver injury caused by rotavirus, and the difference wasstatistically significant (P<0.05). There was no significant difference between theprotein and mRNA expression level of IFN-γ in two groups.Conclusions:1. The cytokines of IL-6, IL-10, TNF-, IFN-γ all play important roles in the processof rotavirus infection. 2. There were significant correlation between the expression levels of IL-6andTNF-and the severity of diarrhea, the infection of rotavirus will be more seriousunder the condition of malnutrition.3. The expression levels of IL-6, IL-10and TNF-were associated with liver injuryinfected with rotavirus.4. The expression levels of IL-10and TNF-were associated with diarrhea. |
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