| Urinary stone is one of the common diseases in the Department of Urology, which has high incidence and recurrence. Currently, the etiology of urinary stone is not very clear, diagnosis and treatment technology developing rapidly, precautions unavailable. Active treatment of urinary (micro) stone to prevent a big stone, or to prevent occurrence and recurrence of urinary stone is very worthy of attention to solve.My tutor, Yonglan Li, created the early diagnosis of urolithisis, the method that can detect urinary microlithiasis through a polarizing microscope, which imaging technology undetect. Professor Yaqiang Zhang, my tutor, has inherited the academic thoughts of Professor Youfang Liu. He made a prescription on the basis of clinical experience, Xiaowei Fangshi Decoction, which has precautionary and therapeutic effects on urinary microlithiasis.Objective To establish the micro calcium oxalate kidney stones of male rats model and to observe the precautionary and therapeutic effects of Xiaowei Fangshi Decoction on the micro calcium oxalate kidney stones of rats and to explore its mechanisms preliminarily.Methods72Wistar rats were divided randomly by random digits table into the normal group, the saline negative control group, the Compound Loosestrife Particles positive control group, the low dose Xiaowei Fangshi Decoction experimental group, the medium dose Xiaowei Fangshi Decoction experimental group, the high dose Xiaowei Fangshi Decoction experimental group. Except those in the normal group, rats in the rest groups received Glycol every morning for5consecutive days. In addition, the saline negative control group, the Compound Loosestrife Particles positive control group, the low dose Xiaowei Fangshi Decoction experimental group, he medium dose Xiaowei Fangshi Decoction experimental group, the high dose Xiaowei Fangshi Decoction experimental group received normal saline, Compound Loosestrife Particles Solution, the low dose Xiaowei Fangshi Decoction, the medium dose Xiaowei Fangshi Decoction, the high dose Xiaowei Fangshi Decoction respectively every afternoon for5consecutive days. After the last drench on the fifth day, the rats in all groups were collected12h urine, weighed, drawed abdominal aortic blood and taken kidneys. Each group of urine was taken the supernatant after centrifugation, and then measured pH, oxalic acid, calcium, creatinine. Each group of abdominal aortic blood was taken the serum after centrifugation, and then measured oxalic acid, calcium, creatinine. Each group of kidneys was weighted, and then made into tissue slices.Results1.The extent of stone in the six groups was statistically significant (P<0.01); Compared with the saline negative control group, the differences in the Compound Loosestrife Particles positive control group and the low dose Xiaowei Fangshi Decoction experimental group were not statistically significant (P>0.05), but the medium dose Xiaowei Fangshi Decoction experimental group and the high dose Xiaowei Fangshi Decoction experimental group were statistically significant respectively(P<0.05, P<0.01); Compared with the Compound Loosestrife Particles positive control group, the differences in the low dose Xiaowei Fangshi Decoction experimental group, the medium dose Xiaowei Fangshi Decoction experimental group and the high dose Xiaowei Fangshi Decoction experimental group were not statistically significant (P>0.05).2.The over all differences of the pH in the urine, the oxalic acid, calcium, creatinine in the urine and the serum in the six groups were not statistically significant respectively(P>0.05); Compared with the normal group and the saline negative control group, the medium dose Xiaowei Fangshi Decoction experimental group and the high dose Xiaowei Fangshi Decoction experimental group were not statistically significant respectively(P>0.05).3.The differences of left kidney weight, right kidney weight and final rat weight were statistically significant respectively(P<0.05). Compared with the normal group and the saline negative control group, the high dose Xiaowei Fangshi Decoction experimental group was statistically significant respectively(P<0.05).Conclusion1.It is successful to establish the micro calcium oxalate kidney stones of male rats model by gavage with Glycol.2.Micro calcium oxalate stones have no significant influences on kidney function. The high dose Xiaowei Fangshi Decoction has some influences on left kidney weight, right kidney weight and final rat weight, and one of the possible reasons is that the high dose Xiaowei Fangshi Decoction is so thick that it has strong stimulation on rats. 3.Xiaowei Fangshi Decoction has precautionary and therapeutic effects on the micro calcium oxalate kidney stones of rats.4.It is maybe the mechanism that Xiaowei Fangshi Decoction makes the calcium oxalate crystals to reduce through the influence on inhibitor, promoter, the attachment and discharge of calcium oxalate crystals, etc, rather than through the influence of the pH in the urine, the oxalic acid, calcium, creatinine in the urine and the serum on the formation of calcium oxalate crystals.5.In some way, the medium dose Xiaowei Fangshi Decoction and the high dose Xiaowei Fangshi Decoction have better precautionary and therapeutic effects on the micro calcium oxalate kidney stones of rats than the Compound Loosestrife Particles. The treatment of urinary micro stones should be Xingqi Huoxue and Jianpi Yishen, besides Qingre Lishi and Liniao Paishi. |
