Analysis The Lymphocytes Subgroup And Cytokines Level Of The Long-term Survival Patients After OLT

BackgroundLiver transplantation has become the most effective treatment of end-stage liver disease is Liver transplantation. Long-term survival rate in liver transplant recipients has also been significantly improved. However, long-term use of immunosuppressive agents will bring a various side effects. Induction of immune tolerance in liver transplant recipients without long-term use of immunosuppressive agents is the key to improving long-term survival rate and quality of their life. Immune tolerance is closely related to various types of immune cells. This article is to explore the characterization of the long-term survival patients through monitoring the lymphocytes subgroup and cytokines level, Intend to find a suitable marker of tolerance from various immune cells in peripheral blood for monitoring and evaluation of recipient’s clinical status.. MethodThe lymphocytes subgroup (T, B, and NK) and cytokines (IL9,17,22) of patients and healthy groups were tested by flow cytometry and Elisa. ResultThe level of CD3+, CD4+, CD8+, CD8+CD28+/CD8+and IL-9were gradually increased in the short-term group and long-term group, were approached to those of healthy group. The percentage of CD3+cell and level of IL-9were significantly lower in short-term group and long-term group than in healthy group (P<0.05); the percentage of CD8+cell was significantly lower in short-term group than in healthy group (P<0.05); the ratio of CD8+CD28+/CD8+was significantly lower in short-term group than in long-term group and healthy group (P<0.05). The level of B cell. NK cell and IL-22were gradually increased in the healthy group, short-term group and long-term group. The percentage of B cell and NK cell were significantly higher in long-term group than in healthy group (P<0.05); the level of IL-22was significantly higher in long-term group than in short-term group and healthy group (P<0.05). However, NKT lymphocytes and IL17was no statistic difference. ConclusionThe ratio of T lymphocyte subgroup and the level of IL-9were good biomarkers for evaluating the immune characterization of OLT patients; NK, B lymphocytes and IL-22maybe associated with the long-term survival in patients after OLT.