| Background and Objective: CD117, as the protein product of kitprotooncogene, is diffused and strongly expressed in most of thegastrointestinal stromal tumor (GIST), but in about4%-15%of GIST,CD117is expressed as weakly positive or negative, which makes itdifficult for the diagnosis of GIST. DOG1is a kind of protein with highexpression and specificity in GIST. Some researches show that itssensitivity is closed to or even higher than CD117, so it is a marker withhigh sensitivity to diagnose GIST and can be used for the supplementarydetection for proving the negative expression of CD117in GIST. Thecombination of DOG1and CD117detections will increase the diagnosticrate of stromal tumor. GIST has a potential malignancy and extremelyhigh recurrence rate after the operation. With the poor prognosis, itsfive-year survival rate is lower than35%, and there has not been anygenerally-accepted biological marker with specificity which can judge themalignant potential of GIST until now. Some documents report that thepositive expression of DOG1is related to GIST size, position, risk degreegrading, etc. It can be regarded as a potential indicator to judge GISTprognosis, but no consensus has been reached at present, and the relationbetween the clinicopathologic characteristics of GIST and DOG1still needs a further research to provide more evidences. Ki67, as a kind ofgene relevant to the cell proliferation, is closely related to thetumorigenesis and progression. Its expression level can reflect theproliferative activity of tumor cell and is often used to judge thebiological behavior and prognosis of malignant tumor. Currently, thereare few researches on the value of Ki67to judge the malignant potentialand prognosis of GIST, and the research results also have differences.This experiment jointly detects the expression levels of DOG1, CD117and Ki67in GIST tissue and incisal edge tissue by theimmunohistochemical SP method, analyzes the relation between DOG1and Ki67as well as the clinicopathologic characteristics of GIST, anddiscusses their effects on diagnosing GIST and judging the malignancygrade and prognosis.Methods: select30clinical cases meeting China’s GIST diagnosticcriteria, and in these cases, all of the patients once received the surgicalresection in the Gastrointestinal Surgery of The Affiliated Hospital ofLuzhou Medical College from May2012to July2013, and they havecomplete clinal data and have no adjuvant therapy history before theoperation. The resected specimens have intact tumor tissue and normalincisal edge tissue. Choose30cases of tumor tissue specimens andnormal incisal edge tissue specimens respectively, and all specimens mustbe confirmed through the pathological examination. Adopt the immunohistochemical SP method to jointly detect the expression levelsof DOG1, CD117and Ki67proteins in30cases of tumor tissuespecimens, then compare the aforesaid levels with the expression levelsin30cases of normal incisal edge tissue specimens, and finally analyzethe correlation between DOG1and Ki67as well as the clinicopathologiccharacteristics of GIST, NIH risk degree grade and prognosis.Result：1. In gastrointestinal stromal tumors tissues the expression rate ofDOG1was90%（27/30）,in incisal edge tissues the expression rate ofDOG1was0%（0/30）,there is a statistically significant differencebetween the two（P＜0.05）. The expression of DOG1in gastrointestinalstromal tumors tissue have no significant difference with age、sex、tumorlocation、tumor size、histological type、tumor mitotic count、NIH riskclassification（P＞0.05）.2. In gastrointestinal stromal tumors tissues the expression rate ofKi67was76.7%（23/30）, in incisal edge tissues the expression rate ofKi67was6.7%（7/30）, there is a statistically significant differencebetween the two（P＜0.05）. The expression of Ki67in gastrointestinalstromal tumors tissue have no significant difference with age、sex、tumorlocation、histological type（P＞0.05）,but have a significant differencewith tumor size、tumor mitotic count、NIH risk classification（P＜0.05）. 3. In gastrointestinal stromal tumors tissues the expression rate ofCD117was86.7%（26/30）, in incisal edge tissues the expression rate ofCD117was0%（0/30）, there is a statistically significant differencebetween the two（P＜0.05）.4. In30cases of GIST tumor tissues: DOG1and CD117expressionwas positive in24cases, there is one case were negative; CD117negative and DOG1positive3cases, CD117positive and DOG1negative2cases. The expression of DOG1and CD117in gastrointestinal stromaltumors tissue have no significant difference（P＞0.05）.5. The expression of DOG1and Ki67in gastrointestinal stromaltumors tissue have no significant difference（P＞0.05）.Conclusion:1. The expression of DOG1in gastrointestinal stromal tumors tissuehave no significant difference with age、sex、tumor location、tumor size、histological type、tumor mitotic count、NIH risk classification.2. DOG1highly expressed in GIST, it is a highly sensitive markersin the diagnosis of GIST. Combined detection DOG1, CD117expressionmay improve the diagnostic yield of GIST. DOG1can not be used as anindicator to determine the malignant potential and prognosis of GIST.3. The expression of Ki67in gastrointestinal stromal tumors tissuehave no significant difference with age、sex、tumor location、histological type,but have a significant difference with tumor size、tumor mitoticcount、NIH risk classification.4. Ki67can be used as an Reference Index to determine themalignant potential and prognosis of GIST.5. The expression of DOG1and Ki67in gastrointestinal stromaltumors tissue have no significant difference. |
PDF Full Text Request