| Objective:Study the pharmacognostical characteristics and inherent quality of the medicinal materials of the stems of Lonicera japonica, provide experimental data and scientific evidence for establishing the quality standards. To study the protective effects of water-soluble extact of the Lonicera japonica stemes on the vascular endothelial cells.Methods:Established the vascular endothelial cells injury model by LPS, added water-soluble parts of the stems of the Lonicera japonica respectively to examine it’s protective effect on the vascular endothelial cells. Detected cell viability by MTT method, cell cycle by Flow Cytometry and Malondialdehyde (MDA), oxygen free radicals (ROS), lipid peroxide (LPO), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), quinone reductase (NADPH), total cellular antioxidant capacity (T-AOC) by biochemical kits. According to the quality standard of the Pharmacopoeia of the Peple’s Republic of China (2010Edition) the water, ash and extract contents of the stems of the Lonicera japonica were measured, and the contents of the Chlorogenic acid and luteolin glycosides were determined with the HPLC.Results:Through the treatment of VEC with10μg/mL LPS and protective drugs, cell survival rate and drug concentration in a dose-dependent effect, when it reaches a certain dose, cell viability began to decline.10μg/mL water-soluble extact can significantly reverse cells damage. There is almost no protection of quercetin, to some extent, increasing cells damage. Experimental data of the cell cycle show that S phase cells decreased significantly in the model group, G1/G0phase and G2/M phase ratio increased. S phase cells increased in the treatment group and the cell cycle returned to normal. It means that cells are capable of DNA replication and begin to undergo mitosis. Malondialdehyde(MDA), oxygen free radicals (ROS) was reduced, lipid peroxide (LPO) activity decreased, while superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), quinone reductase (NADPH) content increased, total cellular antioxidant capacity (T-AOC) enhanced, which could be detected by biochemical kits experimental data after adding water-soluble parts of the stems of Lonicera japonica. The protection of the stems of the Lonicera japonica to VEC were selected:water-soluble extact.19batch of different varieties of the stems of the Lonicera japonica medicinal components content were determined, measured the moisture content is between6.74%～12.52%, the total ash content is between2.68%～3.75%, acid insoluble ash content is between0.18%～0.39%, water soluble extract content is between12.33%～19.82%, alcohol soluble extract content is between15.23%～23.34%, the total phenolic acid content is between3.84%～6.07%, total flavonoids content is between2.14～3.64%, the quality of the chlorogenic acid content is between0.35%～0.80%, mignonette glycosides content is between0.007%～0.048%.Conclusions:Water-soluble parts of the stems of the Lonicera japonica have protective effects on VEC. They reversed the anomalies of lipid peroxidation damage and cellular redox of VEC induced by LPS. It indicated that the protective mechanism may be reversing the anomaly of cell oxidative stress induced by LPS. By measuring, was found in19different species of the stems of the Lonicera japonica medicinal materials in the total ash content, alcohol soluble extract and the chlorogenic acid content in full compliance with the standards of the stems of the Lonicera japonica on the Pharmacopoeia of the Peple’s Republic of China (2010Edition). Moisture content and the moisture related regulation of the stems of the Lonicera japonica on the Pharmacopoeia of the People’s Republic of China (2010Edition),only a sample exceeded standard, suggests he degree of processing should be paid more attention; Acid insoluble ash content, water soluble extract, total phenolic acids, flavonoids and mignonette glycosides content, also laid the foundation for the further study for the development and application of the stems of the Lonicera japonica. |
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