| In this study, the plant growth regulators(gibberellic acid anduniconazole)were used in Lingshou honeysuckle(English name) to regulatethe flowering phase. The proper treatment methods were decided.withmeasuring the flowering phase, related morphological indexs and activeingredients content of honeysuckle which was treated by the plant growthregulators with different spraying concentrations and times. This study canprovide theoretical guidance for the scientific and reasonable honeysucklecultivation techniques.In addition, the content of active ingredients in Lingshou honeysuckleand some commercial species(origin from Henan, Shandong, Anhui and Juludistrict in Hebei) was compared in this research. The active ingredientscontent was also determined in different parts of honeysuckle collected indifferent seasons. This research can provide theoretical basis for the qualityevaluation and resource development of Lingshou honeysuckle.1Methodological research on the content detection of active ingredientsin honeysuckle1.1Methodological research on the content detection of chlorogenic acidand luteoloside in honeysuckleChinese pharmacopoeia in2010was referred and the high performanceliquid chromatography conditions of chlorogenic acid and luteoloside wereoptimized. The results showed that chlorogenic acid and luteoloside had agood linear relationship with the peak area in the range of13.84~147.6μg/mL,14.93~159.2μg/mL and the regression equations were y=35.1x-85.6,r=0.9999and y=27.2x-36.5, r=1.0000, respectively. In this experiment, thedetection limits of chlorogenic acid and luteoloside were13.8×10-6and 56.0×10-6mg/mL, respectively. The quantitation limits were46.1×10-6and187×10-6mg/mL, respectively. In the precision experiment, RSD was less than2.2%and2.6%. For the repeatability experiment, RSD was less than2.3%and1.3%. RSD was less than1.7%and1.9%in the stability experiment. Theaverage recovery was99.4%,98.1%and RSD was less than1.8%and1.5%,respectively. These methods are accurate, reliable, simple and reproducible, sothey are suitable for the content detection of chlorogenic acid and luteoloside.1.2Methodological research on the content detection of total flavonoids inhoneysuckleUV-spectrophotometry method was used to determine the content of totalflavonoids in honeysuckle. Rutin was selected as a kind of reference substanceand NaNO2-Al(NO3)3-NaOH test solution was selected as the chromogenicreagent. The studies showed that rutin had a good linear relationship with theabsorbance in the range of10.82~54.10μg/mL and the regression equationwas y=11.48x-0.0051, r=0.9999. RSD was less than1.6%in the precisiontesting. In the repeatability testing, RSD was less than2.6%. RSD was lessthan1.0%and1.3%in the color compounds and samples stability testings,respectively. The average recovery was99.7%and RSD was less than1.5%.The results showed that this method is simple, convenient, accurate andsuitable for the quality control of total flavonoids in honeysuckle.1.3Methodological research on the content detection of mentalelements(Cu、Zn、Fe、Mg、Ca) in honeysuckleThe samples were digested with mixed acid of HNO3and HClO4by thewet digestion method. FAAS method was established to detect the content ofCu, Zn, Fe, Mg and Ca. The linear range of these five elements was0.375~6.00μg/mL,0.200~1.60μg/mL,2.00~10.0μg/mL,0.0500~0.600μg/mL,1.25~15.0μg/mL and the regression equations were Cu:y=0.0386x-0.0026, r=1.0000; Zn: y=0.2913x-0.0084, r=0.9998; Fe:y=0.0469x+0.0179, r=0.9996; Mg: y=0.5563x+0.0026, r=0.9995; Ca:y=0.0236x+0.0044, r=0.9998, respectively. RSD was less than0.9%,0.9%,1.7%,0.9%and1.0%in the precision testings. In the repeatability testings, RSD was2.9%,1.2%,1.7%,1.5%and2.9%, respectively. RSD was less than0.7%,1.4%,1.3%,1.4%and1.5%in the stability testings, respectively. Theaverage recovery of these five elements was97.5%,101.8%,98.4%,100.9%,101.2%and RSD was less than2.9%,2.6%,2.3%,2.8%and1.8%,respectively. These methods are accurate, reliable, convenient, and can beused to detect the content of Cu, Zn, Fe, Mg and Ca.2Research on flowering regulation of Lingshou honeysuckleIn this study, two kinds of plant growth regulators (gibberellic acid anduniconazole) were used to deal with honeysuckle in the field. The resultsindicated that gibberellic acid can promote the flower bud formation anduniconazole can postpone the florescence. So the entire harvest period couldextend5to6days. Gibberellic acid had no remarkable effect on the buddinglength, fresh weight, dry weight and the content of luteoloside, totalflavonoids, but the content of chlorogenic acid in honeysuckle treated bydifferent concentrations of gibberellic acid was increased significantly. Thetreatment of1000mg/L gibberellic acid solution which was sprayed threetimes could make the chlorogenic acid content increase from2.41%(CK) to2.77%. Compared with the control group, the budding length, fresh weight,dry weight, the content of chlorogenic acid, luteoloside and total flavonoids inhoneysuckle treated by uniconazole could not be improved significantly.3Comparative Study on active ingredients content in Lingshou honeysuckleand some commercial speciesCompared with the commercial species from different habitats (Henan,Shandong, Anhui, Julu district in Hebei), the content of chlorogenic acid andtotal flavonoids in Lingshou honeysuckle was the highest and the content was4.03%and4.27%, respectively. The Luteolin content of Lingshouhoneysuckle (0.120%) was higher than that of commercial varieties(Shandong,Anhui, Julu district in Hebei). The content of some metal elements, such as Znand Fe, was higher in Lingshou honeysuckle than that in others. While thecontent of Cu, Mg and Ca was lower.4Comparative Study on active ingredients content in different parts of honeysuckle collected in different seasonsThe content of active ingredients varied significantly in different partsof honeysuckle. The results showed that the content of chlorogenic acid in thealabastrum was the highest and the content in the stem was the lowest. Theluteoloside and total flavonoids content was the highest in the leaf. Thecontent of Cu and Zn elements in the alabastrum was the highest and therewere plenty of Fe, Mg and Ca in the leaf.The content of active ingredients in the samples collected in differentseasons also varied. The leaf and stem grew vigorously in summer (late May)and the content of active ingredients (such as chlorogenic acid, luteoloside,total flavonoids) and Fe was accumulated largely. So it was appropriate timefor collecting the samples. The content of Zn and Ca in the samples gatherd inwinter (late November) was higher than that in the samples collected in othertime. |
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