The Preliminary Study Of Molecular Mechanisms On Hypoglycemic Effect Of Myricetin And Hypolipidemic Efficacy Of Bamboo Leaves Flavonoids

Flavonoids are a class of polyphenolic natural products from a wide variety of vegetables, fruits and medicinal plants. Modern research have found that flavonoids have a variety of biological activity on antioxidant, lipid regulating, radical scavenging, antibacterial activity, anti-inflammatory, anticancer and hypoglycemic effect, etc. They also have some preventive and therapeutic effects on diabetes, high cholesterol and their complications. Myricetin and bamboo leaves flavonoids become a top research for they have rich source, widely used, high safety performance and significant effect. The preliminary study of molecular mechanisms based on pharmacological activity in hypoglycemic effect of myricetin and lipid-lowering efficacy of bamboo leaves flavonoids was done in this research. It can provide a new theoretical perspective for expanding the development of blood glucose and lipid-lowering products.Many physiological and biochemical parameters were determined by giving different doses of myricetin and bamboo leaves flavonoids respectively to diabetic and hyperlipidemic rats for studying the effects of pharmacological activity. Part of the molecular mechanisms on hypoglycemic effect of myricetin and lipid-lowering efficacy of bamboo leaves flavonoids were reached by quantitative analysis of gene expression of insulin receptor, glucose transporter protein-4, glucose-6-phosphatase, Bax, Bcl-2in diabetic rats, and fatty acid synthase, hormone-sensitive lipase, PPARy in hyperlipidemic rats(1) Reducing blood glucose effectively, improving the body’s glucose tolerance and organ function was found in diabetic rats by continuous injection of myricetin (200mg/kg) two weeks. At the same time, promoting islet β cells to secrete insulin, improving T-SOD enzyme activity, reducing MDA content, increasing the body’s antioxidant properties was found in diabetic rats.(2) Quantitative analysis showed that Myricetin play a role in lowing blood glucose by downregulation gene expression of Bax to promote the regeneration of islet (3cells and secrete insulin; upregulation gene expression of GLUT4and InsR to improve insulin sensitivity and reduce insulin resistance of tissue; suppression gene expression of G-6-pase to slow down the degradation of glucose or glycogen gluconeogenesis and decrease production of glucose(3) The flavonoids from bamboo leaves have significant lipid-lowering effects. After intervention experiment of4weeks, compared with model group, the24%bamboo leaves flavonoids can significantly reduce serum TC, TG, LDL-C levels, increase serum HDL-C levels; the43%bamboo leaves flavonoids can significantly reduce serum TC levels, increase serum HDL-C levels. We also found that bamboo leaves flavonoids of low concentrations are more conducive to reducing serum TC, LDL-C levels in hyperlipidemic rats, and bamboo leaves flavonoids of high concentrations affect serum HDL-C, TG levels greatly.(4) The bamboo leaves flavonoids can work hypolipidemic effect by downregulation gene expression of FAS to reduce synthesis of fat; upregulation gene expression of HSL to increase degradation of fat and control weight of rats; upregulation gene expression of PPARγ to regulate the storage and release of fat, promote transport of lipid, make metabolism of lipid in a benign cycle...