Extraction And Separation And Identification Of Drug-resistant E. Coli Plasmid Medicine

Purpose: Explore the E. coli extract Wenyang Tonglin Soup resistance and molecularmechanisms of drug resistance.Method: Extraction "Wenyang Tonglin Soup" in the main active ingredient is smallmolecules.UPEC clinical isolates.Determination of the minimum inhibitory concentration ofdrug (MIC).Analysis times inhibitory concentration of drug-induced bacterialresistance.Determination of mutation frequency test strain.Selected mutant strain is the mostfrequently used experimental strains.Extraction with alkaline lysis method of plasmid DNA, itis transformed into E. coli competent cells, and to study the transformation of bacteriaresistant.Identification of resistance transfer plasmid by agarose gel electrophoresis.Result: The MIC is the E. coli CP91.0g/ml, inhibition zone diameter of2mm, is highlyresistant to the medicine.Engaging the resistant strains obtained MIC was0.25g/ml, thediameter of the inhibition zone is4mm, the medicine exhibits resistance.Conjugal transferexperiments showed that the DNA fragment size of45kb plasmid can be transferred from E.coli to CP9DH5By plasmid profiles found to pass the engagement before the experiment isnot found in the recipient plasmid DNA in bacteria, but the same was found after conjugaltransfer of plasmid DNA and the size of the donor strain E. coli strains obtained by theengagement contained (45kb).Sensitivity test showed MIC as resistant strains combined0.125g/ml, inhibition zone diameter of3mm.It is suggested that a size of45kb plasmid can betransferred from a donor to a recipient strain of E. coli bacteria Gram-positive Staphylococcusaureus and the average transfer rate of1×10-2transconjugants/acceptor, and aureus plasmidgeneration still maintain resistance.Conclusion: Resistant plasmids were transformed into E. coli CP9can S. aureusRN450RF.The transconjugants acquire plasmid.This plasmid is indistinguishable from the E.coli plasmidby criteria of size. This evidence shows that E. coli plasmidcan replicate and beexpressed in S. aureus.