Tonifying Kidney And Strengthening Spleen And Dampness Removing Blood Stasis Method Of Study On The Effect Of Serum And Tissues TNF-α And IL-6 In Rats With IgA Nephropathy

Purpose: Observation of kidney and spleen, blood with wet method of IgA nephropathy model in rats serum and kidney tissue Tumor necrosis factor alpha, the Influence of Interleukemiakin-6, further study is given priority to with kidney and spleen, blood with wet method of clinical curative effect mechanism in the treatment of Immunoglobulin A nephropathy.Method: Selected 80 healthy clean male Wistar rats, weight(200 +) 20 g, adopt the method of stratified random adaptability will have been feeding 1 week 10 rats were divided into normal group, made 70 module.Reference Liu Huiyu improved Immunoglobulin A nephropathy building method [1].Building modules using bovine serum albumin Bevine serum albumin(BSA) + Carbon tetrachloride( CCL4) + Lipopolysaccharide(LPS)Lipopolysaccharide, the method to establish model of IgA nephropathy rats, a total of 9 weeks.Model group rats to build mode after a successful application of the digital random method is divided into blank group, model group, positive control group(hematuria capsule), the treatment group(citric acid stilbene complex renal capsule high, medium and low dose group).Blank to saline lavage group and model group, positive control group to the blood in the urine capsule to fill the stomach, the treatment group in terms of stilbene after kidney shot to fill the stomach, for 8 weeks. To observe the general condition of rats, detect each group rats each period of 24 mice quantitative urine protein, urine red blood cell count;Before and after treatment were detected in rats of liver and kidney function and biochemical indexes such as;ELISA method is applied to detect serum and kidney tissues of the variation in content of Tumor necrosis factor alpha, Influence of Interleukemiakin-6Result:(1) Imitate Liu Huiyu improved IgA nephropathy building methods, such as, building success finally.Cuhk rat killed 11 experimental process.In the whole experiment process of blank group rats generally in good shape, bright color, activity is normal, normal to the toilet.Model group rats for 5 weeks appear different degree of the colour not gloss, decreased activity, eat bad, body weight, urine yellow, diarrhea, etc.After medication for three weeks,rats gradually improve the performance, in terms of stilbene after kidney pills with hematuria capsule group improved obviously, which dose group of symptoms is most obvious, model group rats not seen significant relief of symptoms.(2) Model group compared with blank group rats urine red blood cell count and a significant rise in 24 hour urine protein quantitative, both comparison was statistically significant(P < 0.05).Treatment group and control group of rats after dosing, urine red blood cell count and 24 hour urinary protein were decreased, quantitative terms of stilbene after kidney bolus group compared with hematuria capsule group was statistically significant(P <0.05).Terms of stilbene after comparison between kidney bolus group was statistically significant(P < 0.05), of which the optimal dose group.(3) The model group compared with the blank group rats Tumor necrosis factor alpha,Interleukemiakin-6 in serum and kidney tissue expression have to rise, after medication Tumor necrosis factor alpha, Influence of Interleukemiakin-6 expression decreased, the treatment group is better than that of control group(P < 0.05), the treatment way between the more significant differences(P < 0.05), of which the optimal dose group.(4) Groups of rat liver and kidney in the treatment of before and after comparison there was no significant difference(P > 0.05).(5) The expression of the IgA, fluorescence microscope, visual renal capsule wall model group, continuous green fluorescent ring accidentally see dotted fluorescence, malpighian tube artery cross-sectional can spread along the artery wall contorts the linear green fluorescence.Conclusion:(1) Improve the method of building completely imitating Liu Huiyu etc animal model to mimic the IgA nephropathy, about 9 weeks model success;(2) Kidney and spleen, lishi blood Chinese medicine can significantly reduce the amount of urine protein and urine red blood cell count, and on the curative effect is better than that of blood in the urine capsule group;Of which the most dose group curative effect is obvious;(3) Compared with blank group, model group rats serum and kidney tissues of Tumor necrosis factor alpha and significantly increased the expression of Interleukemiakin-6, were significantly lower after treatment Tumor necrosis factor alpha and the rise ofInterleukemiakin-6 expression is involved in the pathogenesis of Immunoglobulin A nephropathy;(4) Kidney and spleen, lishi blood Chinese medicine can significantly reduce Tumor necrosis factor alpha in rat serum and kidney tissue and the expression of Interleukemiakin-6,so it can be speculated that kidney and spleen, blood is Chinese traditional medicine with wet by lowering the expression of Tumor necrosis factor alpha and Interleukemiakin-6, achieve the goal of treatment of Immunoglobulin A nephropathy, thereby further protection of renal function.