Effects Of Coix Seed Oil On Proliferation And Expression Of VEGF Of Human Retinal Capillary Endothelial Cells In High Glucose Environment

AIM: To study the effects of different concentrations of Coix seed oil on human retinal capillary endothelial cells( HRCECs) proliferation and vascular endothelial growth factor( VEGF) expressing in high glucose environment.METHODS: HRCECs extracted from human fresher eyeball and cultured in vitro.and ultimately used in the experiment were the growth of 3-4th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose +(50ul/ml, 100ul/ml, 200ul/ml)different concentrations Coix seed oil group.Detecting the multiplication of HRCECs by MTT, The immunocytochemical method was employed to detect the each group HRCECs of VEGF expression.RESULTS: MTT assay results showed that: different concentrations of coix seed oil acted at HRCECs for 48 hours, Inhibition of cell proliferation was significant difference compared with high glucose control group(P<0.05). There was no statistical difference between the low glucose group and high glucose control group(P>0.05).Immunocytochemical assay showed that: 50ul/ml, 100ul/ml, 200ul/ml coix seed oil acted at HRCECs 48 hours,the expression of VEGF decreased significantly compared with the high glucose control group(P<0.05),and in a dose-dependent manner. However, in high glucose control group, the expression of VEGF was obvious higher than that of low glucose control group(P<0.05).CONCLUSION:Coix seed oil can suppress the expression of VEGF of HRCECs in high glucose environment cultured in vitro, which maybe one of important mechanisms to inhibit the proliferation of HRCECs.