Effects Of Gambogic Acid On HRCECs Proliferation And VEGF Expression In High Glucose Environment

Objective:In today's society,diabetic retinopathy has become one of the serious eye diseases that threaten vision and cause blindness.On the one hand,it seriously affects the vision and quality of life of patients,on the other hand,it also brings a huge economic burden to our healthy society.This article starts with in vitro cell experimental studies to explore the effects of gambogic acid on the proliferation of human retinal vascular endothelial cells(HRCECs)and the expression of vascular endothelial growth factor(VEGF)in a high glucose environment.Lesions provide evidenced and valuable data.Methods: Human retinal vascular endothelial cells were isolated from fresh human eyeballs after corneal transplantation and cultured in vitro for experiments.The HRCECs were cultured in a high glucose medium to simulate the internal environment of diabetes.The experiments were performed in the following groups: low glucose control group,Solvent control group,high-sugar control group,high-sugar plus different concentrations of gambogic acid group,according to the pre-experiment results and previous references,the concentration gradients of gambogic acid drugs established were 2?mol / L,4?mol / L,8?mol / L,The effect of gambogic acid on the proliferation of HRCECs under different drug concentrations and different action times was measured by thiazole blue colorimetry(MTT),and the effect of VEGF expression in HRCECs was detected by Western Blot.The time points detected by MTT and Western Blot methods were 12,24,and 36 hours,respectively.Results: 1.The MTT method showed that there was no significant difference in absorbance A between the solvent control group and the high glucose control group(P> 0.05).In the low-glucose group,the high-sugar group can promote the proliferation of HRCECs.With the increase of time,the effect of promoting cell proliferation is more obvious.In the high-sugar environment,the proliferation ability of HRCECs cells is inhibited by gambogic acid,and it shows a time-dose concentration dependence,that is,Gambogic acid can inhibit the proliferation of HRCECs under high glucose environment at 2?mol / L,4?mol / L,and 8?mol / L(P <0.05).As time goes by and the drug concentration increases,its ability to inhibit proliferation also increases.Strengthening,36 hours after the effect,the cell proliferation was inhibited most obviously.2.Western Blot showed that there was no significant difference in the VEGF gray value between the solvent control group and the high glucose control group(P> 0.05).Compared with the low glucose group,the high glucose environment promoted the expression of VEGF in HRCECs,and the difference was statistically significant.Significance(P <0.05).With time,the gray value of VEGF in the high-glucose group increased gradually;compared with the high-glucose group,different concentrations of gambogic acid in the high-glucose group(2?mol / L,4?mol / L,8?mol / L)The gray value of VEGF decreased,and the difference was statistically significant(P <0.05);at the same time point,the high glucose group with different concentrations of gambogic acid drug treatment group(2?mol / L,4?mol / L,8?mol / L)VEGF The gray values were compared with each other,and the difference was statistically significant(P <0.05),Moreover,with the extension of time,the gray level of VEGF decreased with the same concentration of drug intervention.In high glucose environment,gambogic acid significantly down-regulated the expression of VEGF and showed a time-dose concentration-dependent effect for 36 hours.The high concentration group(8 ?mol / L group)had the most significant effect on inhibiting VEGF expression.Conclusion: 1.Different concentrations of gambogic acid showed significant inhibition of HRCECs proliferation in vitro under high glucose environment,and showed a time-concentration dependent relationship.2.When HRCECs were cultured in a high-glucose environment in vitro,more VEGF was produced than in a low-glucose environment.Gambogic acid could inhibit the expression of HRCECs VEGF in a high-glucose environment in vitro in a time-concentration dependent manner.