Analysis Of The Risk Factors Of Nonalcoholic Fatty Liver In Han Population Receiving Physical Examination Of Guilin And Gene Polymorphism In Promoter Region Of Adiponectin Gene

Objective: To investigate the prevalence and risk factors of nonalcoholic fatty liver(NAFLD) in Han physical examination population of Guilin and the association of SNPs-11377 C/G in proximal promoter region of adiponectin gene with NAFLD. Methods: 6660 persons were selected from medical center for health examination by cluster sampling method and examined. The survey included questionnaire investigation, height, weight and blood pressure check-up, alanine aminotransferase(ALT), aspartate aminotransferase(AST), fasting blood glucose(FPG), triglyceride(TG), total cholesterol(TC), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), uric acid(UA) and liver color doppler ultrasound examination, and body mass index(BMI) calculated. The adiponectin gene 11377 C/G genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method in 260 subjects, including 130 controls and 130 NAFLD patients. Results:(1) The crude prevalence of NAFLD in the examination population of Guilin was 19.1% and 16.9% standardized(male 24.8%, female 12.9%, the age-standardized 23.4% and 10.1%). The prevalence of NAFLD was significantly higher in male than female before the age of 50 and significantly higher in female than male in the age of 70. The prevalence of age crosspoint was in 50 ~ 69 years old.(2) As BMI increased,the prevalence of NAFLD had significant increasing trend. NAFLD in overweight and obesity group were obviously higher than normal group among the physical examination receivers(P<0.01), and NAFLD in obesity group was significantly higher than in overweight group(P<0.01).(3) NAFLD was detected in 1273 physical examination receivers(male 862 receivers, female 411 receivers), and healthy people were selected by age and sex matched with NAFLD group as the normal control group. The clinical data of two groups were analyzed: BMI、systolic blood pressure(SBP)、diastolic blood pressure(DBP)、ALT、AST、FPG、TG、TC、LDL-C and UA in NAFLD group were significantly higher than in normal control group(P<0.01), HDL-C level was lower in NAFLD group than in normal control group(P<0.01).(4) Logistic regression anaIysis demonstrated that sex(male)、BMI、blood pressure、ALT、FPG、TG、LDL-C and UA were the risk factors of NAFLD, and HDL-C was the protective factor of NAFLD.(5) Analysis of SNPs-11377 C/G in proximal promoter region of adiponectin gene: The adipenectin genotypes(CC: 59.2% vs 63.8%, CG: 36.2% vs 30.8%, GG: 4.6% vs 5.4%) and allelic frequencies(C: 77.3% vs 79.2%, G: 22.7% vs 20.8%) were not significant differences between NAFLD and normal control groups(P>0.05). Compared with clinical variables of adipenectin different genotype(CC and CG/GG), two groups were also not significant differences(P>0.05). Conclusions:(1) Substantial proportions of physical examination population of Guilin suffered from NAFLD, and there existed a tendency for young people involved; the prevalence of NAFLD had increasing trend with BMI.(2) NAFLD was positively correlated with metabolic syndrome(MS).(3) Sex(male)、BMI、blood pressure、ALT、FPG、TG、LDL-C and UA were the risk factors of NAFLD, and HDL-C was the protective factor of NAFLD.(4)There is no obvious association between SNPs-11377C/G in proximal promoter region of adiponectin gene and NAFLD in Han population receiving physical examination of Guilin.