Effect Of Gingko Biloba Extract On The Blood Pressure,Heart, Kidney And Vessel Of Intermittent Hypoxia Rats

Objective To observe the effect of intermittent hypoxia and chronic hypoxia on rat blood pressure, and to investigate the function of Gingko biloba extract(GBE) on obstructive sleep apnea syndrome(OSAS) with hypertension in rats. Methods 50 healthy male Sprague-Dawley(SD) rats were randomly divided to 5 groups(N=10):normal control(NC) group, chronic intermittent hypoxia(CIH) group, chronic intermittent hypoxia with GBE intragastric administration group, chronic hypoxia, chronic hypoxia with GBE intragastric administration group. The change of blood pressure was observed before and after experiment.The pathological changes about myocardia, renal artery and abdominal artery were observed by hematoxylin(HE) staining.The content of TNF-α 、 IL-1、IL-6、MDA, 4-HNE and HIF-1α were tested in serum. The level of TLR4, JNKprotein and its expression of mRNA were detected in rat adipose tissue. Results①The tail arterial systolic pressure showed that: Blood pressure of CHI group was significant increased than that in NC group(P < 0. 01). In intermittent hypoxia with GBE intragastric administration group, the blood pressure was lower than that in CIH group(P<0. 01). Compared with NC group, there had no statistical significance in intermittent hypoxia with GBE intragastric administration group, chronic intermittent hypoxia group and chronic hypoxia with GBE intragastric administration group(P >0. 05). ②The result of HE stating showed that: Compared with NC group, the myocardial cells were significant swelling and kidney tubules hydropic degeneration in CIH group and chronic intermittent hypoxia group. In intermittent hypoxia with GBE intragastric administration group and chronic hypoxia with GBE intragastric administration group, the myocardial cell and kidney tubules were not so swelling compared with CIH and chronic hypixia group. The pathological change of abdominal artery in each group had no significant different. ③ELISA showed that: The levels of TNF-α,IL-1 and IL-6 in CHI were significant higher than those in NC(P<0.01),and the content was lower in GBE compared with CHI(P<0.05),But the results about TNF-α,IL-6 in GBE had no statistical significance compared with NC,and the content of IL-1βwas higher than that in NC(P<0.01). the content of TNF-α in serum of chronic hypoxia group was significantly higher compared with NC group(P<0.05), while the content of IL-1β、IL-6 had no difference; The contentof TNF-α in serum of chronic hypoxia group and GBE gavage group’ serum was lower than chronic hypoxia group P < 0. 01);Compared with NC group, the content in serum of MDA, 4-HNE, HIF-1α in serum were significant higher in CIH group and chronic hypoxia group(P<0.05). However, the results of them in intermittent hypoxia with GBE intragastric administration group, chronic hypoxia with GBE intragastric administration group were lower compared with CIH and chronic hypoxia group(P<0.05). ④Western-blot and RT-PCR showed that: Compared with NC group, the level of toll-like receptor4(TLR4), JNK protein and its expression of mRNA were significant increased in adipose tissue of the CIH and chronic hypoxia group(P<0.01). But in intermittent hypoxia with GBE intragastric administration group, chronic hypoxia with GBE intragastric administration group, they were lower than CIH and chronic hypoxia group(P<0.01). Conclusion Intermittent hypoxia could elevate the blood pressure in rats, but chronic hypoxia couldn’t cause this. GBE could restrain the expression of TLR4, JNK protein and its mRNA in adipose tissue of OSAS and chronic hypoxia rats, and it also could reduce the level of TNF-α 、 IL-1β 、 IL-6 、MDA,4-HNE,HIF-1α in serum, and reduce hydropic degeneration in cardiomyocytes, kidney tubules water samples degeneration. So we conclude that,hypoxia(intermittent or chronic) may result in abnormal inflammatory reaction and oxidative stress, Intermittent hypoxia could elevate the blood pressure in rats, but chronic hypoxia couldn’t cause this.GBE can be used as an effectivedrug to treat and prevent the secondary hypertension caused by OSAS, and it can alleviate the damage of hypoxia on organs and tissues.