Effect Of Agaricus Blazei Murrill On Typeâ…¡diabetes Mellitus In Mice

Objective:To observe the effect of agaricus blazei murrill(ABM) on glucose and lipid metabolism in typeⅡdiabetes mellitus(T2DM) model mice and to analyze the mineral elements in ABM.Methods:Among 75 male ICR mice, 15 mice were randomly selected as a control group, and the remaining mice were used to induce non-insulin-dependent diabetes mellitus(T2DM) by intraperitoneal injection of 100mg/kg streptozocin(STZ). After two days, the mice injected STZ were randomly divided into four groups: model group, ABM low, middle and high dose group with 1%,5%,10% of ABM, respectively. The control group and model group fed normal mice fodder and all groups fed distilled water lasted for 8 weeks. During the experiment, body weight, food intake, water intake were weekly recorded. Fasting blood glucose were measured before STZ injection and in the middle and at the end of the experiment. At end of the experiment, the serum insulin, lipids and other biochemical markers were measured by kits. The pancreatic tissues were used to observe islet morphology and performed immunocytochemistry analysis.In addition, Cr, Zn, Se, Mn, Cu and other elements in ABM were determined using inductively coupled plasma mass spectrometry(ICP-MS).Results:1.The animal experimentAfter intraperitoneal injection of STZ, the mice showed obvious polydipsia, polyphagia, polyuria. Body weight also decreased in model mice.At the end of experiment, fasting blood glucose was significantly lower in ABM low, middle and high dose groups than that in model group. The level of serum insulin was higher in ABM groups than model group, but there was no significant differences were observed. The homeostasis model assessment-% β(HOMA-%β) index, which reflects the islet β cell secretory function, were calculated using fasting blood glucose and fasting insulin.This index were signifycantly higher in ABM groups than that in model group. Serum low density lipoprotein cholesterol(LDL-C) were lower in ABM groups without significant difference from model groups.Pancreatic morphology with HE staining indicated that shrank islets and decreased number of islets in model group. Nuclear fusion and necrosis of β cells were also observed. According to the immunocytochemistry analysis, insulin secretion decreased in model group. While, the islet morphology in ABM groups improved as well as insulin secretion function, especially in the middle and high dose group.2.The trace elements in ABMThe contents of Cr, Zn, Se, Mn, Cu in ABM were 0.529±0.128, 60.596±4.579, 1.004±0.144, 11.582±0.130, 52.132±0.418 mg/kg, respectively.Conclusion:The present study successfully induced T2 DM model using 100mg/kg STZ. ABM supplementation decreased fasting blood glucose, increased insulin levels and improved glucose metabolism in T2 DM mice. The effect of ABM was notobvious on lipid metabolism. The trace elements in ABM were likely relate to the development of T2 DM.