| Objective Use ozone water point injection in the treatment of ankylosing spondylitis points and methods of observation model of collagen induced arthritis rats serum TNF alpha, the influence of IL- 6, explore the ozone water to the mechanism of action of acupuncture point injection treatment of ankylosing spondylitis.Methods 1 Select 40 clean male wistar rats, according to the digital random method is divided into four groups, namely blank control group, model control group, ozone water group, etanercept group, normal 7 days later, according to the following method to dispose: blank control group will not be any processing;In addition to the blank control group in the rest of the rats using Ⅱ collagen type and incomplete freund’s adjuvant mixed emulsifying subcutaneous injection mold, namely collagen induced arthritis(CIA) rat model.2 The end of the building, blank control group, model control group does not give any interference, ozone water group to select the following point: "Dazhui", "Fengchi", "Jiaji", "Geshu", "Shenshu", "Dachangshu" and to give acupuncture point injection of ozone water; etanercept group was given subcutaneous injections.Twice a week for 3 weeks treatment.3 Each group after the treatment cycle in rats by intraperitoneal injection of chloral hydrate anesthesia in rats after plate fixation, by abdominal aorta to take blood, blood after centrifugal collection group, the serum with ELISA kit each rat serum content of TNF alpha, IL- 6 in.Results1 The performance of the experimental group rats appearanceModel control group rats left toes after gradually after the inflammatory swelling, weight lifting, swelling, and local skin reddening, swelling and thickening, touch, there was an obvious escape behavior of rat activity is difficult, fur color dim;Peak, degree of swellingin the 21 st day fade, weight increases slightly;Ozone water group and etanercept group and model control group before treatment of rats rats is basically the same performance, after treatment, reduced the symptoms of rats, with the progress of the treatment cycle, further reduce the symptoms of the treatment group rats, and eating well, weight increasing, performance is more active, fur gradually luster. 2 The rats toes swelling degree after leftModel control group compared with blank control group increased significantly, the difference was statistically significant(P < 0.05); Ozone water, according to the heap group compared with model control group were significantly lower, the difference was statistically significant(P < 0.05); Ozone water compare differences between the groups with and in accordance with the heap has no statistical significance(P > 0.05).3 Rats serum TNF alpha, IL- 6 in comparison Model control group were significantly increased compared with blank control group, the difference was statistically significant(P < 0.05); Ozone water group compared with model control group were significantly lower, the difference was statistically significant(P < 0.05); In accordance with the heap group compared with model control group were significantly lower, the difference was statistically significant(P < 0.05); Ozone water group and in accordance with the heap comparative differences had no statistical significance(P > 0.05).Conclusion Ozone water point injection is able to cut the inflammatory cytokine TNF alpha, the levels of IL- 6 and inhibit rat toes swelling degree, shows that the treatment can inhibit the development of collagen induced arthritis disease. |
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