| Background: Epilepsy is a commom disease of the central nervous system and not only the pathogenesis is extremely complicated, but also the pathogenesis has not been precisely defined. These diseases repeat frequently and complicated symptoms, which will lead to seriously cognitive disorder and impact the patients’ work and normal life and generally lead to the physical and mental aberrance, even invalid and death. Currently most AEDs on the market have a single mechanism for treating EP following so many side effects, and drug resistance also will reduce the function of AEDs. Therefore, it is nessary to for us to develop new AEDs. Ginkgo Biloba Extract(EGb) contains kinds of substance, such as flavone glycoside, ginkgolide and organic acids. It was suggested that EGb could i the EP and improve patients’ cognition in many ways.Objective: In the article, we established the temporal lobe epilepsy rats induced by lithium-pilocarpine to observe the spatial learning and memory abilities and investigate the effects of EGb on the behavior and pathologic changes of the epilepsy rats.Methods:(1) The temporal lobe epilepsy rats induced by lithium-pilocarpine would be divided into five groups, they are model group(physiologic saline); high doses group(80mg/kg EGb); middle doses group(60mg/kg EGb); low doses group(40mg/kg EGb); positive group(108mg/kg CBZ);and control group(eating normally). All the groups had 5 rats. The electroencephalography of every group rats was recorded by Power Lab.(2) The temporal lobe epilepsy rats induced by lithium-pilocarpine will be divided into five groups, they are model group(physiologic saline); high doses group(80mg/kg EGb); middle doses group(60mg/kg EGb); low doses group(40mg/kg EGb); positive group(108mg/kg CBZ);and control group(eating normally). All the groups had 10 rats. The spatial learning and memory abilities of every group were tested by using Morris, and HE straining and Nissl straining was used to observe the change of brain in the rats, then Western blotting and immunohistochemistry was used to evaluate the expression of BDNF, GDNF, VEGF in the rat hippocampus of every groups.(3)Pimary cell culture was used to obtain neural stem cell, and specialized immunochemical procedure was used to evaluating the neural stem cell. To compare the differentiation-inducing effect of EGb in neural stem cell, we determined the neural stem cell by specialized immunochemical procedure.Results:(1) For control group, electroencephalogram of rats was mainly α or β waves with small amount of θ waves, without obvious rhythmic. However, the model group showed a diffuse epileptiform waves, mostly spike wave, sharp wave, slow spines composite wave, and showed a large number of epileptiform discharge; High, middle and low doses group showed the scattered spike wave, sharp wave.(2) Compared with the control group, the spatial learning and memory abilities of model group were significantly reduced. For Model group, a large number of neurons were lost in hippocampus. However, compared with the model group, the spatial learning and memory abilities of rats administrated with EGb were significantly improved, and the neurons in hippocampus were increased. On the other hand, the results of Western blotting and immunohistochemistry was demonstrated that the expression of BDNF,GDNF,VEGF was increased in hippocampus.(3) Primitive cultivation of neural stem cells, the cells gathered nerve ball formation, increase with the increase of number of days; After passage, a small amount of cells could adherence, natural differentiate. Immunofluorescence staining showed that nerve ball was nestin positive, which demonstrated the cells was the NSC. Serum and ginkgo biloba extract could effectively induce neural stem cells, which are stronger than the natural differentiation, but high levels of ginkgo biloba extract could induce cell death; Immune staining showed that it was NSE positive after cell differentiation suggesting that neural stem cells was differentiated into neurons.Conclution:(1) Ginkgo biloba extract could effectively improve the abnormal epileptiform discharge in brain of epileptic rat;(2) Ginkgo biloba extract could significantly improve the spatial learning and memory abilities in lithium-pilocarpine-induced status epilepticus rats, and could significantly prevent the neuronal loss, increase the expression of BDNF,GDNF,VEGF;(3) Ginkgo biloba extract could effectively promote neural stem cells to differentiate into neurons, and improve the loss of hippocampus neuron to achieve the effect of treatment of epilepsy. |
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